摘要
目的探索5种不同含量的聚己内酯/还原氧化石墨烯(Polycaprolactone/reduced graphene oxide,PCL/RGO)神经导管基底膜材料与PC12细胞的体外生物相容性。方法应用静电纺丝技术制备5种不同RGO含量的PCL/RGO神经导管基底膜样材料,并与PC12细胞共培养。使用CCK-8法测定PC12细胞的增殖情况,扫描电镜观察细胞的黏附与生长情况,活死细胞染色观察细胞活性。结果5组材料所对应的细胞增殖率有差异,但毒性分级均为0级(无细胞毒性),其中0.75%RGO/PCL组的细胞增殖情况最佳。5组材料培养7 d后,电镜观察显示细胞黏附生长明显较1 d、3 d增多,并铺满了材料表面。活死细胞染色结果显示,各组细胞存活率均在90%以上。结论5种不同RGO含量的PCL/RGO复合材料均具有良好的生物相容性,尤以0.75%RGO/PCL在促进细胞增殖、黏附方面有比较大的优势。
Objective To explore the in vitro biocompatibility of five different contents of polycaprolactone/reduced graphene oxide(PCL/RGO)nerve duct basement membrane materials with PC12 cells.Methods Five kinds of PCL/RGO nerve duct basement membrane materials with different RGO contents were prepared by electrospinning technique and cocultured with PC12 cells.The proliferation of PC12 cells was determined by CCK-8 method,the adhesion and reproduction of PC12 cells were observed by scanning electron microscopy,and the cell activity was observed by Live/Dead fluorescence staining.Results The cell proliferation rate of the five groups was different,but the toxicity grade was 0(no cytotoxicity),and the cell proliferation of 0.75%RGO/PCL group was the best.After 5 groups of materials were cultured for 7 days,the cell adhesion growth was significantly increased under electron microscope compared with 1 day and 3 days,and the surface of the materials was fully coated.The Live/Dead fluorescence staining results showed that the survival rate of cells in all groups was above 90%.Conclusion Five kinds of PCL/RGO composites with different RGO contents all have good biocompatibility,and the composite materials with 0.75%RGO/PCL ratio have a relatively large advantage in promoting cell proliferation and adhesion.
作者
叶开
黄晓夏
仵博宇
王江华
乌日开西·艾依提
何乐乐
滕勇
YE Kai;HUANG Xiaoxia;WU Boyu;WANG Jianghua;Wurikaixi·Aiyidi;HE Lele;TENG Yong(Graduate School of Xinjiang Medical University,Urumqi 830000,China;Department of Spinal Surgery,General Hospital of Xinjiang Military Region,PLA,Urumqi 830000,China;School of Mechanical Engineering,Xinjiang University,Urumqi 830000,China)
出处
《组织工程与重建外科》
CAS
2024年第4期416-421,共6页
Journal of Tissue Engineering and Reconstructive Surgery
基金
新疆维吾尔自治区区域协同创新专项(科技援疆)计划(2019E0277)
国家自然科学基金(51565057)。
