摘要
目的探讨壁虎多肽混合物(GPM)对人非小细胞肺癌H1299细胞增殖和凋亡的影响。方法实验设正常对照组(等体积基础培养液)和GPM高、低剂量组(20,10 mg/mL),采用CCK-8法检测细胞增殖情况,采用流式细胞术检测细胞凋亡情况,采用实时荧光定量聚合酶链反应(RT-qPCR)法和免疫印迹(Western blot)法检测B-细胞淋巴瘤因子2(Bcl-2)、Bcl-2关联X蛋白(Bax)、磷脂酰肌醇-3-激酶(PI3K)mRNA及蛋白表达水平。结果与正常对照组比较,GPM高、低剂量组H1299细胞存活率均显著降低(P<0.01),细胞凋亡率均显著升高(P<0.01),PI3K和Bax mRNA及蛋白表达水平均显著降低(P<0.01),Bcl-2 mRNA及蛋白表达水平均显著升高(P<0.05)。结论GPM能抑制H1299细胞的增殖,其作用机制可能与调控PI3K,Bax,Bcl-2 mRNA及蛋白的表达相关。
Objective To investigate the effect of gecko peptides mixture(GPM)on the proliferation and apoptosis of human non-small cell lung cancer(NSCLC)H1299 cells.Methods The experiment was conducted with the normal control group(equal volume of basic culture medium),high-dose and low-dose GPM groups(20,10 mg/mL).CCK-8 method was used to detect cell proliferation.The flow cytometry was used to detect cell apoptosis.Real-time fluorescence quantitative polymerase chain reaction(RT-qPCR),Western blot were used to detect the expression levels of B-cell lymphoma factor 2(Bcl-2),Bcl-2 associated X protein(Bax),and phosphatidylinositol 3-kinase(PI3K)mRNA and protein.Results Compared with those in the normal control group,the cell survival rate of H1299 cells significantly decreased(P<0.01),while the cell apoptosis rate significantly increased in the high-dose and low-dose GPM groups(P<0.01).The expression levels of PI3K and Bax mRNA and protein significantly decreased(P<0.01),while the expression levels of Bcl-2 mRNA and protein significantly increased in the high-dose and low-dose GPM groups(P<0.05).Conclusion GPM can inhibit the proliferation of H1299 cells,and its mechanism may be related to the regulation of the PI3K,Bax,Bcl-2 mRNA and protein expression.
作者
姚志新
刘嘉欣
朱桂芬
黄慧贤
陈少娜
YAO Zhixin;LIU Jiaxin;ZHU Guifen;HUANG Huixian;CHEN Shaona(The First Affiliated Hospital of Guangzhou University of Chinese Medicine·Guangdong Clinical Research Academy of Chinese Medicine,Guangzhou,Guangdong,China 510405)
出处
《中国药业》
CAS
2024年第17期61-65,共5页
China Pharmaceuticals
基金
广东省中医药局科研项目[20221152]。
