摘要
为了深入理解淫羊藿(Epimedium brevicornu Maxim.)治疗类风湿性关节炎(rheumatoid arthritis,RA)的作用机制,采用网络药理学与分子对接技术进行分析。通过数据库及相关软件获取淫羊藿和RA的交集靶点蛋白,构建蛋白质-蛋白质相互作用(protein-protein interaction,PPI)网络和“药材-成分-靶点”互作网络,对靶点蛋白进行富集分析,最后进行蛋白分子对接。结果表明,淫羊藿治疗RA的核心靶点蛋白可能为TNF、AKT1、TP53、ALB、SRC等;有效活性成分可能为β-谷甾醇、8-异戊烯基山奈酚、谷甾醇、山奈酚、木樨草素、槲皮素等;信号通路可能为癌症中的信号通路、脂质和动脉粥样硬化等;分子对接结果显示淫羊藿治疗RA的有效活性成分与靶点蛋白具有极好的结合活性。该研究证明淫羊藿治疗RA具有多成分、多靶点、多通路的特点,为实验研究和临床治疗提供了坚实的理论基础。
To gain a deeper understanding of the mechanism by which Epimedium brevicornu Maxim.treats rheumatoid arthritis(RA),network pharmacology and molecular docking techniques for analysis were employed.The intersection target proteins of E.brevicornu Maxim.and RA through databases and related software were obtained,the protein-protein interaction(PPI)network and″medicinal material-component-target″interaction network were constructed,the target proteins were enriched and analyzed,and protein molecule docking was performed.The results indicated that the core target proteins of E.brevicornu Maxim.for treating RA might be TNF,AKT1,TP53,ALB,SRC,etc.The active ingredients might beβ-sitosterol,8-isopentenyl kaempferol,sitosterol,kaempferol,luteolin,quercetin,etc.The signal pathway might be the signal pathway in cancer,lipid,and atherosclerosis.The molecular docking showed that the active ingredients of E.brevicornu Maxim.for treating RA had excellent binding activity with target proteins.The results demonstrated that E.brevicornu Maxim.,in the treatment of RA,possessed characteristics of multiple components,multiple targets,and multiple pathways,providing a solid theoretical foundation for its experimental research and clinical treatment.
作者
兰德淼
姚锦坤
嵇常青
肖强
LAN Demiao;YAO Jinkun;JI Changqing;XIAO Qiang(Hubei Key Laboratory of Biological Resources Protection and Utilization,Enshi 445000,China;College of Forestry and Horticulture,Hubei Minzu University,Enshi 445000,China)
出处
《湖北民族大学学报（自然科学版）》
CAS
2024年第3期330-336,共7页
Journal of Hubei Minzu University:Natural Science Edition
基金
国家自然科学基金项目(31260057)
湖北省科技厅技术创新重大专项资助项目(2019ACA120)
湖北省自然科学基金项目(2023AFD077)。
关键词
淫羊藿
类风湿性关节炎
网络药理学
分子对接
作用机制
有效活性成分
Β-谷甾醇
Epimedium brevicornu Maxim.
rheumatoid arthritis
network pharmacology
molecular docking
action mechanism
active ingredients
β-sitosterol
