摘要
目的探讨microRNA-132及其靶基因NAG-1在评估强直性脊柱炎(AS)患者非甾体类抗炎药治疗效果中的预测价值。方法选择在该院就诊的AS患者154例作为观察组,抽取同期在该院体检的健康受试者154例作为对照组。观察两组受试者miR-132和NAG-1 mRNA表达水平。观察组患者采用双氯芬酸钠治疗16周,根据美国风湿病学协会(ACR)制定的ACR20标准,将观察组达到标准患者分为ACR20组,其余患者归为非ACR20组,比较两组治疗前miR-132和NAG-1mRNA表达水平及其对疗效的预测价值。结果观察组患者单核细胞miR-132 mRNA表达水平高于对照组,NAG-1 mRNA表达水平低于对照组,差异均有统计学意义(P<0.05)。miR-132预测AS的灵敏度为83.8%,特异性为98.1%,最佳截断值为1.373,曲线下面积(AUC)为0.964,95%CI为0.936~0.982;NAG-1预测AS的灵敏度为76.0%,特异度为86.4%,最佳截断值为0.826,AUC为0.879,95%CI为0.838~0.914。ACR20组患者miR-132mRNA表达水平低于非ACR20组患者,NAG-1mRNA表达水平高于非ACR20组患者,差异均有统计学意义(P<0.05)。miR-132预测ACR20的敏感度为81.8%,特异性为94.2%,AUC为0.950,95%CI为0.903~0.979;NAG-1预测ACR20的敏感度为97.0%,特异性为46.3%,AUC为0.751,95%CI为0.675~0.817。结论miR-132及NAG-1可能是诊断AS及预测非甾体类抗炎药治疗效果的潜在生物学指标。
Objective To investigate the predictive value of microRNA-132 and its target gene NAG-1 in evaluating the therapeutic effect of nonsteroidal anti-inflammatory drugs in patients with ankylosing spondylitis(AS).Methods 154 patients with AS in our hospital were selected as the observation group,and 154 healthy subjects who underwent physical examination in our hospital in the same period were selected as the control group.The expression levels of miR-132 and NAG-1 mRNA in the two groups were observed.Patients in the observation group were treated with diclofenac sodium.The expression levels of miR-132 and NAG-1 mRNA before treatment were observed to predict the efficacy of American Rheumatology Association(ACR)20 after treatment.Results The expression level of miR-132 mRNA in mononuclear of patients in the observation group was higher than that in the control group,and the expression level of NAG-1 mRNA was lower than that in the control group(P<0.05).The sensitivity of miR-132 in predicting AS was 83.8%,the specificity was 98.1%,the best cut-off value was 1.373,the area under the curve(AUC)was 0.964,and the 95%CI was 0.936-0.982.The sensitivity of NAG-1 in predicting AS was 76.0%,the specificity was 86.4%,the best cut-off value was 0.826,AUC was 0.879,and 95%CI was 0.838-0.914.The expression level of miR-132 mRNA in ACR20 group was lower than that in non ACR20 group,and the expression level of NAG-1 mRNA was higher than that in non ACR20 group(P<0.05).The sensitivity of miR-132 in predicting ACR20 was 81.8%,the specificity was 94.2%,the AUC was 0.950,and the 95%CI was 0.903-0.979.The sensitivity of NAG-1 to predict ACR20 was 97,0%,the specificity was 46.3%,the AUC was 0.751,and the 95%CI was 0.675-0.817.Conclusions miR-132 and NAG-1 may be potential biological indicators of the therapeutic effect of AS and non steroidal anti-inflammatory drugs.
作者
张连锁
蔡金生
王会旺
孙亚东
宁胜华
徐伟坤
ZHANG Lian-suo;CAI Jin-sheng;WANG Hui-wang;SUN Ya-dong;NING Sheng-hua;XU Wei-kun(Department of Orthopedics,Xingtai General Hospital,North China Medical and Health Group,Xingtai,Hebei 054000,China)
出处
《颈腰痛杂志》
2024年第4期634-638,共5页
The Journal of Cervicodynia and Lumbodynia
关键词
微小RNA
非甾体类抗炎药激活基因
强直性脊柱炎
非甾体类抗炎药
microRNA
nonsteroidal anti-inflammatory drug activating gene
ankylosing spondylitis
non steroidal anti-inflammatory drugs
