摘要
【目的】探究侧耳属木腐真菌的生物学特性及最佳栽培条件。【方法】以甘南藏族自治州洮河国家级自然保护区的一种野生侧耳属木腐真菌为试验材料,采用组织分离法,分离纯化野生侧耳,获得菌丝体纯培养物,基于形态学和分子生物学方法对其进行分类学研究,并对其进行生物学特性研究。【结果】经鉴定,该野生侧耳为冷杉侧耳(Pleurotus abieticoa)。菌丝体在15、20、25、30℃下均能正常生长,其中25℃生长最佳;在pH为5.0、6.0、7.0、8.0、9.0中均能生长,最适pH为7.0;在供试碳源中,菌丝体在玉米淀粉中生长最快,乳糖中生长最慢;在供试氮源中,蛋白胨中生长最适,尿素中停止生长。【结论】该菌种的最适培养温度为25℃,最适pH为7.0,最佳碳源为玉米淀粉,最佳氮源为蛋白胨。
【Objective】To study the biological characteristics and the best cultivation conditions of Pleu⁃rotus.【Method】In this study,a wood-decaying fungus of Pleurotus collected from the Taohe National Nature Reserve of Gannan Tibetan Autonomous Prefecture was used as the test material.The pure culture was isolated by tissue separation,and the biological characteristics were studied.【Result】The fungus was identified as Pleurotus abieticoa and the mycelium was able to grow normally at the test temperature,and the most suitable being 25℃.It can also grow in the test pH,of which the optimum pH is 7.0.The fungus grows corn starch grows fastest and lactose grows slowest in the test carbon source.Peptone is the most suitable for the growth of the test nitrogen source mycelium,urea does not grow in the test nitrogen source.【Conclusion】The optimum culture temperature of this species was 25℃,the optimum pH was 7.0,the best carbon source was corn starch,and the best nitrogen source was protein.
作者
陈炳
秦永梅
杨礼学
范龙飞
CHEN Bing;QIN Yongmei;YANG Lixue;FAN Longfei(Shandong Agriculture and Engineering University,Jinan 250100,China;Gansu Taohe National NatureReserve Management and protection Center,Zhouni 747600,China;Gansu Agricultural University,Lanzhou 730070,China)
出处
《甘肃农业大学学报》
CAS
CSCD
北大核心
2024年第4期219-224,235,共7页
Journal of Gansu Agricultural University
基金
甘肃农业大学人才引进科研启动基金项目(GAU-KYQD-2021-26)
甘肃省高校青年博士基金项目(2022QB-080)。
关键词
侧耳属
组织分离
形态学
分子生物学
生物学特性
Pleurotus
tissue isolation
morphology
molecular biology
biological characteristics
