摘要
为了提高玉米芯的转化效率,通过正交试验研究了氯化胆碱/乳酸、氯化胆碱/尿素两种低共熔溶剂不同预处理条件对玉米芯酶解的影响.研究结果表明:氯化胆碱/乳酸的预处理条件为预处理温度140℃、固液比1∶15和预处理时间1 h时,总还原糖质量浓度最大为2.860 g/L.三个因素对氯化胆碱/乳酸预处理的玉米芯酶解影响的主次顺序为预处理时间、固液比和预处理温度,三个因素对酶解影响均不显著.氯化胆碱/尿素的预处理条件为预处理温度140℃、固液比1∶20和预处理时间2 h时,总还原糖质量浓度最大为2.941 g/L.三个因素对氯化胆碱/尿素预处理的玉米芯酶解影响的主次顺序为预处理温度、预处理时间和固液比,预处理温度对酶解影响极显著,而预处理时间和固液比对酶解影响不显著.不同的低共熔溶剂和预处理条件对玉米芯酶解的影响是不同的.本研究将为木质纤维素生物质的高值化利用提供理论依据.
In order to improve the conversion efficiency of corncob,the effects of different pretreatment conditions of two deep eutectic solvents,choline chloride/lactic acid and choline chloride/urea,on the enzymatic hydrolysis of corncob were investigated through orthogonal experiment.The results indicated that the maximum total reducing sugar concentration was 2.860 g/L when the pretreatment conditions of choline chloride/lactic acid were pretreatment temperature of 140℃,solid-liquid ratio of 1∶15,and pretreatment time of 1 h.The primary and secondary order of the effects of three factors on the enzymatic hydrolysis of corncob pretreated with choline chloride/lactic acid was pretreatment time,solid-liquid ratio,and pretreatment temperature.All three factors had no significant impact on enzymatic hydrolysis.The maximum total reducing sugar concentration was 2.941 g/L when the pretreatment conditions of choline chloride/urea were pretreatment temperature of 140℃,solid-liquid ratio of 1∶20,and pretreatment time of 2 h.The primary and secondary order of the effects of three factors on the enzymatic hydrolysis of corncob pretreated with choline chloride/urea was pretreatment temperature,pretreatment time,and solid-liquid ratio.The pretreatment temperature had a significant impact on enzymatic hydrolysis,while pretreatment time and solid-liquid ratio had no significant impact.The effect of different deep eutectic solvents and pretreatment conditions on the enzymatic hydrolysis of corncob varied.This study would provide a theoretical basis for the high-value utilization of lignocellulosic biomass.
作者
崔茂金
白国明
何卓汉
李智勇
赵二庆
CUI Maojin;BAI Guoming;HE Zhuohan;LI Zhiyong;ZHAO Erqing(School of Chemistry and Chemical Engineering,Henan Institute of Science and Technology,Xinxiang 453003,China)
出处
《河南科技学院学报（自然科学版）》
2024年第5期8-13,共6页
Journal of Henan Institute of Science and Technology(Natural Science Edition)
基金
河南省科技攻关项目(232102240011)
河南科技学院大学生创新训练项目(2023CX075)
河南科技学院博士科研启动基金项目(210010611001)。
关键词
玉米芯
低共熔溶剂
氯化胆碱
预处理
正交试验
酶解
corncob
deep eutectic solvent
choline chloride
pretreatment
orthogonal experiment
enzymatic hydrolysis
