摘要
目的研究桃红四物汤(Taohong Siwu Tang,TSD)对子宫异常出血(abnormal uterine bleeding,AUB)大鼠血清脂类代谢物的影响,分析TSD改善AUB脂质代谢紊乱的作用机制。方法采用药物不完全流产法复制AUB大鼠模型,应用UPLC-Q-Exactive Orbitrap/MS技术检测血清的脂质代谢物,结合主成分分析、正交偏最小二乘-判别分析筛选差异脂质,明确TSD干预前后血清中脂质变化。结果共筛选出11种差异性脂质,主要有磷酯酰肌醇、磷脂酸、磷脂酰乙醇胺、磷脂酰丝氨酸、固醇脂、神经酰胺、丙烯醇脂及脂肪酸。给予TSD干预后,筛选的差异脂质均有回调至正常的趋势。结论TSD改善AUB可能与磷脂酸、磷酯酰肌醇、磷脂酰乙醇胺、磷脂酰丝氨酸、神经酰胺等脂质代谢有关。
Aim To study the effects of Taohong Siwu Tang(TSD)on serum lipid metabolites in rats with abnormal uterine bleeding(AUB),and to analyze the mechanism of action of TSD in improving lipid metabolism disorders in AUB.Methods The rat model of AUB was replicated by the method of incomplete abortion with drugs,and the lipid metabolites of serum were detected by applying UPLC-Q-Exactive Orbitrap/MS technology,and combined with the principal component analysis and orthogonal partial least squares-discriminant analysis to screen for differential lipids,the changes of lipids in serum before and after the intervention of TSD were clarified.Results A total of 11 differential lipids were screened,mainly phosphatidyl inositol,phosphatidic acid,phosphatidyl ethanolamine,phosphatidyl serine,sterol lipids,ceramide,acrylolipids and fatty acids.The screened differential lipids all tended to regress to normal after the administration of TSD intervention.Conclusion Improvement of AUB by TSD may be related to lipid metabolism such as phosphatidic acid,phosphatidyl inositol,phosphatidyl ethanolamine,phosphatidyl serine,and ceramide.
作者
苏梦宇
张艳艳
黄荣
程瑶
钱珊珊
彭灿
彭代银
刘晓闯
SU Meng-yu;ZHANG Yan-yan;HUANG Rong;CHENG Yao;QIAN Shan-shan;PENG Can;PENG Dai-yin;LIU Xiao-chuang(the First Affiliated Hospital of Anhui University of Chinese Medicine,Hefei 230031,China;School of Pharmacy,Anhui University of Chinese Medicine,Hefei 230012,China;the Second Affiliated Hospital of Anhui University of Chinese Medicine,Hefei 230061,China)
出处
《中国药理学通报》
CAS
CSCD
北大核心
2024年第9期1649-1657,共9页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助项目(No 82104513)
安徽省高校科研项目(No 2023AH050743)。
