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水环境中隐孢子虫检测分析方法与分布特征

Detection and analysis methods of Cryptosporidiumin water environments and distribution characteristics
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摘要 基于隐孢子虫卵囊结构、虫种/基因型和介水传播路径,围绕水环境样本中隐孢子虫的分离纯化、浓度检测、虫种识别、活性判定和感染性评价,综述了相关技术方法,并对其优缺点、适用性进行了归纳总结.基于文献中全国60个地区2667份水环境样品的隐孢子虫分析数据,系统梳理了我国不同地区、不同环境水体中隐孢子虫检出率、浓度等赋存分布特征.结果表明:隐孢子虫在不同地区和不同水体类型中的赋存特征存在显著差异,总体检出率高达32.8%,表明这一公共卫生隐患不容忽视. This study reviews and summarizes the relevant technical methods for Cryptosporidium isolation and purification,concentration detection,species identification,viability assessment,and infectivity evaluation in water environment samples,focusing on the oocyst structure,species/genotypes,and waterborne transmission pathways.The study also compiles the advantages,disadvantages,and applicability of these methods.Based on the analysis data of Cryptosporidium from^(2)667 water environment samples across 60 regions nationwide,the study systematically reviews the occurrence and distribution characteristics,such as detection rates and concentrations,of Cryptosporidium in different regions and types of water bodies in China.The results indicate significant differences in the occurrence characteristics of Cryptosporidium in different regions and water body types,with an overall detection rate as high as 32.8%,highlighting a major public health risk that cannot be overlooked.
作者 邹树斌 肖丹 王楠 陈振 郑添予 范伟 ZOU Shu-bin;XIAO Dan;WANG Nan;CHEN Zhen;ZHENG Tian-yu;FAN Wei(School of Environment,Northeast Normal University,Changchun 130117,China;Jilin Academy of Agricultural Sciences,Changchun 130033,China;Jilin Provincial Academy of Animal Husbandry and Veterinary Medicine,Changchun 130062,China)
出处 《东北师大学报(自然科学版)》 CAS 北大核心 2024年第3期148-156,共9页 Journal of Northeast Normal University(Natural Science Edition)
基金 国家重点研究发展计划项目(2023YFC3706700) 国家自然科学基金面上项目(52270089,52370032,51978135) 长春市科技发展计划项目(21ZY30) 吉林省农业科学院创新工程项目(KYJF2023DX020).
关键词 隐孢子虫 介水传播 检测 分布 Cryptosporidium waterborne transmission detection distribution.
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