摘要
探究益生菌发酵黄芪(fermented Astragali Radix,F-As)与黄芪(Astragali Radix,As)对脂多糖(lipopolysaccharide,LPS)诱导的肠道细胞NCM-460氧化损伤的保护作用及其分子机制。利用MTT法确定了LPS、As和F-As的工作浓度;分别检测并比较了LPS组、As+LPS组和F-As+LPS组,细胞中氧化应激相关指标、抗氧化相关基因在转录水平和蛋白水平表达的变化。与Con组相比,LPS组的活性氧(reactive oxygen species,ROS)和丙二醛(malondialdehyde,MDA)增加(P<0.01),而细胞的存活率、谷胱甘肽(glutathione,GSH)、超氧化物歧化酶(superoxide dismutase,SOD)、总抗氧化能力(total antioxidant capacity,T-AOC)以及细胞中的抗氧化基因在转录水平和蛋白水平均显著降低(P<0.01);而As+LPS组和F-As+LPS组,细胞中的ROS和MDA水平降低(P<0.01),细胞的存活率GSH、SOD、T-AOC以及抗氧化基因在转录和蛋白水平得到恢复(P<0.01),且与As+LPS组相比,F-As+LPS组恢复得更显著(P<0.05)。研究结果提示,As和F-As均可以通过激活抗氧化基因的表达,清除细胞内过多的ROS和MDA,从而对LPS诱导的细胞氧化损伤起到保护作用,且F-As优于As。
This study aims to explore the protective effects and molecular mechanisms of probiotic fermented Astragali Radix(F-As)and non-fermented Astragali Radix(As)on the oxidative damage in lipopolysaccharide(LPS)-induced normal human colon epithelial cell NCM-460.The working concentrations of LPS,As and F-As were determined by MTT assay.The changes of oxidative stress-related indexes and antioxidant-related genes at transcription level and protein level in LPS group,As+LPS group and F-As+LPS group were detected and compared respectively.Compared with the control group,reactive oxygen species(ROS)and malondialdehyde(MDA)were significantly increased(P<0.01)after treatment of LPS,while cell viability,glutathione(GSH),superoxide dismutase(SOD),total antioxidant capacity(T-AOC)and antioxidant-related genes were decreased(P<0.01).Compared with the LPS group,As+LPS group and F-As+LPS group,the content of ROS and MDA were obviously decreased(P<0.01),whereas the content of GSH,SOD,T-AOC and antioxidant-related genes were increased(P<0.01),compared with the LPS group.However,compared with As group,F-As showed a more significant effect on enhancing these bio-indicators as mentioned above(P<0.05).The results suggest that both As and F-As can remove excessive ROS and MDA in cells by activating the expression of antioxidant genes,thus playing a protective role in LPS-induced oxidative damage of cells,and F-As is superior to As.
作者
王萍
王长福
韩士林
匡海学
王秋红
WANG Ping;WANG Chang-fu;HAN Shi-lin;KUANG Hai-xue;WANG Qiu-hong(Key Laboratory of Basic and Application Research of Beiyao Ministry of Education,Heilongjiang University of Chinese Medicine,Harbin 150040,China;Guangdong Engineering Technology Research Center of Traditional Chinese Medicine of Chinese Materia Medica,Guangdong Pharmaceutical University,Guangzhou 510006,China)
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2024年第9期1464-1471,共8页
Natural Product Research and Development
基金
国家重点研发计划中医药现代化研究重点专项(2018YFC1707100)
2022年全国名老中医药专家传承工作室建设项目(国中医药人教函[2022]75号)
黑龙江省教育厅“头雁”团队支持项目(黑龙江省头雁行动领导小组文件[2019]5号)。
关键词
黄芪
益生菌发酵
脂多糖
NCM-460细胞
氧化损伤
Astragali Radix
probiotic ferment
lipopolysaccharide
NCM-460 cells
oxidative damage
