摘要
为揭示辣椒疫霉菌对丛枝菌根真菌(AMF)在辣椒根系定殖能力以及专性内生细菌(MRE)在宿主AMF促进辣椒抗病害胁迫中的潜在作用,选择摩西斗管球囊霉(Fm)、明根孢囊霉(Rc)和幼套球囊霉(Ce)3种与MRE共生的AMF和根内根孢囊霉(Ri)无MRE的AMF种类为试材,通过盆栽建立AMF与辣椒植株的共生定殖体系,然后采用挑战接种方法将辣椒疫霉菌接种于辣椒根系周围,并检测根系中几丁质酶和苯丙氨酸解氨酶(PAL)活性的变化。结果表明,辣椒疫霉菌能够影响AMF在辣椒根系的侵染定殖,不同种类AMF在辣椒根系侵染定殖能力不同,挑战接种辣椒疫霉菌6 d后,MRE共生的Fm、Rc、Ce对辣椒植株的侵染率显著高于无MRE共生的Ri,AMF定殖的辣椒根系中几丁质酶与PAL活性均较对照显著增强;挑战接种辣椒疫霉菌6 d后,接种MRE共生的Fm、Rc和Ce的辣椒几丁质酶活性比接种Ri的分别提高了10.54%、17.00%和13.20%,PAL活性则分别提高了12.49%、3.84%和10.03%。MRE与AMF共生并相互作用,可能是造成不同种类AMF在宿主植物根部侵染率以及诱导宿主抗病害胁迫等方面差异的潜在因素。
To reveal the potential effects of Phytophthora capsici on the colonization ability of arbuscular mycorrhizal fungi(AMF)in pepper roots and the role of Mollicutes/Mycoplasma-related endobacteria(MRE)in promoting pepper resistance to disease stress in host AMF,three AMF species symbiotic with MRE,namely Funneliformis mosseae(Fm),Rhizophagus clarus(Rc),and Claroideoglomus etunicatum(Ce),as well as one AMF species without MRE,Rhizophagus intraradices(Ri),had been selected as test materials.A symbiotic colonization system between AMF and pepper plants had been established through pot cultivation.Subsequently,P.capsici had been inoculated into the rhizosphere of pepper plants using the challenge inoculation method,and changes in the activities of chitinase and phenylalanine ammonia lyase(PAL)in pepper roots had been detected.The results had indicated that P.capsici could affect the colonization of AMF in pepper roots,and different AMF species had varying colonization abilities in pepper roots.After challenging inoculation with P.capsici for 6 days,the colonization rates of Fm,Rc,and Ce symbiotic with MRE in pepper plants had been significantly higher than that of Ri without MRE symbiosis.The activities of chitinase and PAL in pepper roots colonized by AMF had been significantly enhanced compared to the control.After challenging inoculation with P.capsici for 6 days,the chitinase activities in pepper plants inoculated with Fm,Rc,and Ce symbiotic with MRE had increased by 10.54%,17.00%,and 13.20%,respectively,compared to those inoculated with Ri.The PAL activities had increased by 12.49%,3.84%,and 10.03%,respectively.The symbiosis and interaction between MRE and AMF may be potential factors contributing to differences in the colonization rates of different AMF species in host plant roots and their ability to induce host resistance to disease stress.
作者
赵兴丽
阮仕琴
陶刚
娄璇
严雪艳
ZHAO Xingli;RUAN Shiqin;TAO Gang;LOU Xuan;YAN Xueyan(Biotechnology Institute of Guizhou Province,Guiyang 550009,Guizhou,China;College of Eco-Environmental Engineering,Guizhou Minzu University,Guiyang 550025,Guizhou,China;Guizhou Key Laboratory of Agriculture Biotechnology,Guiyang 550009,Guizhou,China;Mingde College,Guizhou University,Guiyang 550031,Guizhou,China)
出处
《中国蔬菜》
北大核心
2024年第9期77-83,共7页
China Vegetables
基金
国家自然科学基金项目(31860520)
黔农科院国基后补助项目([2021]37号)
贵州喀斯特山区重要作物生物育种平台建设项目(黔科合中引地[2023]033)。
关键词
丛枝菌根真菌
专性内生细菌
定殖
侵染率
几丁质酶
苯丙氨酸解氨酶
arbuscular mycorrhizal fungi
Mollicutes/Mycoplasma-related endobacteria
colonization
colonization rate
chitinase
phenylalanine ammonia lyase
