摘要
鹿茸菇菌丝采用液体摇瓶培养,分别测定菌丝生物量、菌丝球数量、菌丝球直径、发酵液pH值、还原糖和氨基氮质量浓度,淀粉酶酶活性、纤维素酶酶活性、漆酶酶活性等生理指标。结果表明:鹿茸菇培养过程中菌丝生物量和菌丝球数量在第10天达到最大值,分别为2.33,106 CFU·mL^(-1),菌丝球直径随培养时间延长而持续变大;还原糖与氨基氮质量浓度先增加而后逐渐降低;淀粉酶及纤维素酶酶活性最大值分别为0.17,0.10 nkat,漆酶胞外酶活性呈现明显上升趋势;摇瓶培养第8~10天的液体菌种生物量达到最大(0.42 mg·mL^(-1)),液体菌种培养终点应控制在第8~10天,此时菌种活力最高,可用于发酵罐扩繁培养。
The mycelia of Lyophyllum decastes is cultured in liquid shake flasks.The physiological indexes such as mycelium biomass,the mycelium ball numbers,the mycelium ball diameters,the pH value of fermentation broth,the mass concentrations of reducing sugar and amino nitrogen,the enzymatic activities of amylases,cellulases and laccases are measured separately.The results show that the mycelium biomass and the mycelium ball numbers both reach the maximum values which are 2.33 mg·mL^(-1)and 106 CFU·mL^(-1)respectively on the 10^(th) day.The mycelium ball diameter continues to increase with the extension of cultivation time.The mass concentrations of reducing sugars and amino nitrogen increase firstly and then decrease gradually.The maximum enzyme activities of amylase and cellulase are 0.17 and 0.10 nkat respectively,while the laccase extracellular enzyme activity increases significantly.The biomass of liquid bacterial strains reaches maximum of 0.42 mg·mL^(-1)on the 8^(th)-10^(th) day in shake flask cultivation,and the end point of the liquid culture should be controlled at the 8^(th)-10^(th) when the activity of the strain is the highest at this time and it can be used for fermenter propagation culture.
作者
彭汇涵
于海玲
王明元
杨苗
万雨欣
PENG Huihan;YU Hailing;WANG Mingyuan;YANG Miao;WAN Yuxin(College of Chemical Engineering,Huaqiao University,Xiamen 361021,China)
出处
《华侨大学学报（自然科学版）》
CAS
2024年第5期667-672,共6页
Journal of Huaqiao University(Natural Science)
基金
福建省厦门市科技计划项目(3502Z20226030)
福建省星火项目(2021S0036,2022S0050)。
关键词
鹿茸菇
液体培养
还原糖
胞外酶
Lyophyllum decastes
liquid culture
reducing sugar
extracellular enzyme
