摘要
为了探究蓟马取食对苜蓿防御酶活性及相关基因的影响,以草原4号紫花苜蓿(Medicago sativa L.cv.Caoyuan No.4)为材料,分别在紫花苜蓿生长59 d前(分枝期)的0 d(对照)、7 d和14 d接种牛角花齿蓟马(Odontothrips loti),测定叶片的防御酶活性,并结合转录组数据分析其编码基因的表达量变化。结果表明:接种蓟马后苜蓿叶片脂氧合酶(LOX)、苯丙氨酸解氨酶(PAL)和多酚氧化酶(PPO)活性显著升高(P<0.05),取食第14 d比对照分别升高了133.30%、120.80%和25.47%;过氧化物酶(POD)和谷胱甘肽还原酶(GR)活性在蓟马取食后第14 d最高,分别比对照升高了93.60%(P<0.05)和11.90%;超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性在蓟马取食后第7 d最高,分别比对照显著升高了82.60%和18.60%(P<0.05)。转录组数据发现编码POD的14个基因中的13个、编码PAL的14个基因中的11个、编码LOX的17个基因中的12个以及编码PPO的全部基因在蓟马取食后表达量均显著上调;而蓟马取食对编码SOD、GR和GSH-Px的不同基因的表达影响不同。苜蓿可能通过调节自身防御酶活性变化及相关编码酶基因的表达应对蓟马取食为害。
This study investigated the impact of feeding by the thrips species,Odontothrips loti,on defense enzyme activity and gene expression in alfalfa(Medicago sativa L.cv.Caoyuan No.4).Plants at the 59-day growth stage(branching stage)were inoculated with thrips,and defense enzymes activities and gene expression levels using transcriptome data were analyzed at 0 day(control),7 days and 14 days post inoculation(dpi).The results showed that thrips feeding significantly increased the activities of Lipoxygenase(LOX),Phenylalanine ammonia-lyase(PAL)and Polyphenol oxidase(PPO)(P<0.05)in alfalfa leaves,compared to the control.At 14 dpi,these enzymes activities were elevated by 133.30%,120.80%,and 25.47%,respectively.Peroxidase(POD)and Glutathione reductase(GR)activities also peaked at 14 dpi,showing increases of 93.60%(P<0.05)and 11.90%compared to the control.Superoxide dismutase(SOD)and Glutathione peroxidase(GSH-Px)activities reached their maximum at 7 dpi,with significant increases of 82.60%and 18.60%compared to the control(P<0.05).Moreover,transcriptome analysis revealed significant upregulation of genes encoding defense enzymes following thrips feeding.Specifically,13 out of 14 POD encoding-genes,11 out of 14 PAL encoding-genes,12 out of 17 LOX encoding-genes,and all PPO encoding-genes showed increased expression.However,thrips feeding had varied effects on the expression of genes encoding SOD,GR and GSH-Px.In conclusion,these findings suggest that alfalfa responds to thrips feeding by regulating both the activity of the defense enzymes and the expression levels of their corresponding genes.
作者
霍晓伟
爽爽
李建伟
张岩
李煜龙
傅清文
张志强
HUO Xiaowei;SHUANG Shuang;LI Jianwei;ZHANG Yan;LI Yulong;FU Qingwen;ZHANG Zhiqiang(College of Grassland Science,Inner Mongolia Agricultural University/Technology Engineering Center of Drought and Cold-Resistant Grass Breeding in North of the National Forestry and Grassland Administration/Key Laboratory of Grassland Resources of the Ministry of Education/Key Laboratory of Forage Cultivation,Processing and High Efficient Utilization of Ministry of Agriculture and Rural Affairs/Inner Mongolia Key Laboratory of Grassland Management and Utilization,Hohhot 010011,China)
出处
《中国草地学报》
CSCD
北大核心
2024年第9期34-42,共9页
Chinese Journal of Grassland
基金
内蒙古自治区高等学校“青年科技英才支持项目”(NJYT23009)
内蒙古自治区自然科学基金面上基金项目(2021SHZR0721)
国家自然科学基金项目(32160333)
高等教育改革发展项目——研究生科研创新资助项目(S20231109Z)。
