摘要
目的探究幽门螺杆菌(helicobacter pylori,Hp)对慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)大鼠模型肺组织病理变化及黏蛋白5AC(mucin5AC,MUC5AC)表达的影响。方法选取33只Wistar大鼠,将其随机分为空白组、COPD组、Hp组、COPD+Hp组,其中空白组、COPD组、Hp组大鼠各9只,COPD+Hp组6只。COPD组、COPD+Hp组采用香烟烟熏联合气管内滴注脂多糖法;Hp组、COPD+Hp组采用Hp灌胃法。模型建立成功1个月后,收集各组大鼠肺组织,行苏木精-伊红(hematoxylin-eosin,HE)染色观察肺组织病理,阿利新蓝-过碘酸雪夫(Alcian blue periodic acid-Schiff,AB-PAS)染色观察气道杯状细胞增生情况,免疫组化法检测MUC5AC蛋白的表达水平,实时荧光定量聚合酶链反应(real-time fluorescence quantitative polymerase chain reaction,RT-qPCR)检测MUC5AC mRNA表达的水平。结果与空白组比较,COPD组、Hp组、COPD+Hp组大鼠气道单位长度杯状细胞计数、肺组织MUC5AC蛋白和mRNA表达明显增加,差异有统计学意义(P<0.05);与COPD+Hp组比较,COPD组、Hp组大鼠气道单位长度杯状细胞计数、肺组织MUC5AC蛋白和mRNA表达降低,差异有统计学意义(P<0.05)。结论Hp感染加重了COPD的炎性反应和气道杯状细胞增生,加重COPD的气道分泌和MUC5AC的表达,促进了COPD的进展。
Objective To investigate the effects of helicobacter pylori(Hp)on pathological changes and mucin5AC(MUC5AC)expression in lung tissue of rats with chronic obstructive pulmonary disease(COPD).Methods Thirty-three Wistar rats were randomly divided into blank group(n=9),COPD group(n=9),Hp group(n=9),COPD+Hp group(n=6).Cigarette smoking combined with intratracheal instillation of lipopolysaccharide was used in COPD group and COPD+Hp group,and Hp intragastric administration was used in Hp group and COPD+Hp group.The establishment of the model after one month,the lung tissues of rats were collected,the pathology of lung tissue was observed by hematoxylin-eosin(HE)staining,the proliferation of airway goblet cells was observed by Alcian blue periodic acid-Schiff(AB-PAS)staining,the expression level of MUC5AC protein was detected by immunohistochemistry,and the expression level of MUC5AC mRNA was detected by real-time fluorescence quantitative polymerase chain reaction(RT-qPCR).Results Compared with the blank group,the goblet cell count per unit length of airway,the expression of MUC5AC protein and mRNA in lung tissue in COPD group,Hp group and COPD+Hp group were significantly increased,and the differences were statistically significant(P<0.05).Compared with COPD+Hp group,the goblet cell count per unit length of airway,the expression of MUC5AC protein and mRNA in lung tissue in COPD group and Hp group were lower,and the differences were statistically significant(P<0.05).Conclusion Hp infection aggravates the inflammatory reaction and the proliferation of goblet cells in the airway of COPD,increases the airway secretion and the expression of MUC5AC,and promotes the progression of COPD.
作者
王兆霞
戈改真
丁瑞峰
梁利娜
WANG Zhaoxia;GE Gaizhen;DING Ruifeng(Graduate School of Baotou Medical College,Inner Mongolia University of Science and Technology,Inner Mongolia 014040,China;不详)
出处
《医学研究杂志》
2024年第9期86-90,97,共6页
Journal of Medical Research
基金
内蒙古自治区自然科学基金资助项目(2020MS08069)。
