摘要
烟草是重要的经济作物,烟草青枯病对烟草产业造成了极大的经济损失。因此需要一种安全有效的方法防止烟草青枯病的发生。前期研究中发现可溶性钙肥和生防菌均能有效抑制青枯病菌的生长。在田间种植烟草时,通过单施或组合施用不同的含钙土壤调理剂和生防菌,筛选防治烟草青枯病的最佳组合;采集不同处理的烟草根际土,运用荧光定量PCR和高通量测序技术,探究不同处理下烟草根际土壤中青枯菌数量的变化以及微生物群落组成和结构的变化。田间试验表明,土壤调理剂和生防菌的施用均能有效降低烟草青枯病的发生,但3种含钙调理剂中只有硝酸铵钙能更好地与生防菌GT11复合。该组合处理能降低土壤中青枯病菌的数量,改善烟草根际土壤微生物的群落组成与结构,显著降低烟草青枯病的发病率和病情指数,田间防效达60%。研究证实硝酸铵钙与贝莱斯芽孢杆菌GT11的联合施用能够有效防治烟草青枯病,为烟草生产中青枯病的防治提供了新的思路和理论依据。
Tobacco is one of the most important cash crops,and bacterial wilt of tobacco causes a great loss to tobacco production.Therefore,a safe and effective method is needed to prevent the occurrence of tobacco bacterial wilt.In the previous study,it found that the soluble calcium fertilizer could effectively control tobacco bacterial wilt.When tobacco was planted in the field,the best combination was selected by applying different soil conditioner containing calcium and biocontrol bacteria.The quantity of Ralstonia solanacearum was quantified through fluorescence quantitative PCR.The composition and structure of microbial community in tobacco rhizosphere soil under different treatments were studied by high-throughput sequencing.Field experiments showed that the application of soil conditioner and biocontrol agent could effectively reduce the occurrence of tobacco bacterial wilt,but only ammonium calcium nitrate could compound with biocontrol bacteria GT11 better.The combination treatment decreased the quantity of bacterial wilt in soil,improved the composition and structure of microbial community in tobacco rhizosphere soil,and significantly reduced the incidence and disease index of tobacco bacterial wilt.The control effect in the field was up to 60%.The results showed that the combination of calcium ammonium nitrate and Bacillus velezensis GT11 could effectively control tobacco bacterial wilt,which provided a new idea and theoretical basis for tobacco production.
作者
王博文
王金星
邱成尧
秦庆宪
王菊香
姚博
李红艳
谭官鑫
杨应伟
姬广海
WANG Bowen;WANG Jinxing;QIU Chengyao;QIN Qingxian;WANG Juxiang;YAO Bo;LI Hongyan;TAN Guanxin;YANG Yingwei;JI Guanghai(College of Plant Protection,Yunnan Agricultural University,Kunming 650201,China;Yunnan Province Tobacco Company Puer Company,Yunnan Tobacco Company,Yunnan Puer 665000,China)
出处
《生物技术进展》
2024年第5期839-847,共9页
Current Biotechnology
基金
景谷烟区烟草青枯病综合防控技术研究与应用(PEYCKY202201)。
