摘要
目的探讨线粒体肌酸激酶1A(CKMT1A)、细胞视黄酸结合蛋白2(CRABP2)、配对盒基因8(PAX8)在子宫内膜癌患者血清中的表达水平及其与临床病理参数和预后的关系。方法选取2021年5月至2022年5月唐山市妇幼保健院收治的107例子宫内膜癌患者作为研究组。并在治疗后进行1年定期随访,根据患者复发情况分为复发组和未复发组。另选取同时期在该院进行体检的86例健康者作为健康组。采用酶联免疫吸附试验检测各组血清CKMT1A、CRABP2、PAX8水平,比较不同临床病理指标的子宫内膜癌患者血清CKMT1A、CRABP2、PAX8水平。采用Pearson相关分析子宫内膜癌患者血清CKMT1A、CRABP2、PAX8水平的相关性。绘制受试者工作特征(ROC)曲线分析血清CKMT1A、CRABP2、PAX8单独及三者联合检测对子宫内膜癌复发的预测价值。结果研究组血清CKMT1A、CRABP2、PAX8水平均高于健康组,差异均有统计学意义(P<0.05)。Pearson相关分析结果显示,血清CKMT1A与CRABP2水平呈正相关(r=0.437,P<0.001),CKMT1A与PAX8水平呈正相关(r=0.526,P<0.001),CRABP2与PAX8水平呈正相关(r=0.493,P<0.001)。不同肌层浸润、分化程度、淋巴结转移情况等的子宫内膜癌患者血清CKMT1A、CRABP2、PAX8水平比较,差异均有统计学意义(P<0.05),且血清CKMT1A、CRABP2、PAX8水平随着分化程度的降低及淋巴结的转移而升高(P<0.05)。复发组纳入12例患者,非复发组纳入95例患者。复发组血清CKMT1A、CRABP2、PAX8水平均高于非复发组,差异均有统计学意义(P<0.05)。ROC曲线分析结果显示,血清CKMT1A、CRABP2和PAX8单独预测子宫内膜癌复发的曲线下面积分别为0.886、0.850、0.811,均低于三者联合检测的0.978(Z三者联合-CKMT1A=2.318,P=0.020;Z三者联合-CRABP2=2.030,P=0.042;Z三者联合-PAX8=2.955,P=0.003)。结论子宫内膜癌患者血清CKMT1A、CRABP2和PAX8水平均高表达,三者表达与病理类型、分化程度、淋巴结转移情况等临床病理参数以及预后有关,可以作为评估子宫内膜癌患者预后的生物学指标。
Objective To investigate the expression levels of mitochondrial creatine kinase 1A(CKMT1A),cellular retinoic acid binding protein 2(CRABP2)and paired box gene 8(PAX8)in serum of patients with endometrial carcinoma and their relationship with clinicopathological parameters and prognosis.Methods A total of 107 patients with endometrial cancer admitted to Tangshan Maternal and Child Health Hospital of Hebei Province from May 2021 to May 2022 were selected as the study group.The patients were followed up for 1 year after treatment,and were divided into recurrence group and non-recurrence group according to the recurrence.In addition,86 healthy people who underwent physical examination in the hospital during the same period were selected as the healthy group.Enzyme-linked immunosorbent assay was used to detect the serum levels of CKMT1A,CRABP2 and PAX8 in each group,and the serum levels of CKMT1A,CRABP2 and PAX8 in endometrial cancer patients with different clinicopathological indicators were compared.Pearson correlation was used to analyze the correlation of serum CKMT1A,CRABP2 and PAX8 levels in patients with endometrial cancer.The receiver operating characteristic(ROC)curve was drawn to analyze the predictive value of serum CKMT1A,CRABP2,PAX8 alone and their combined detection for the prognosis and recurrence of endometrial cancer.Results The serum levels of CKMT1A,CRABP2 and PAX8 in the study group were higher than those in the healthy group,and the differences were statistically significant(P<0.05).Pearson correlation analysis showed that serum CKMT1A level was positively correlated with CRABP2 level(r=0.437,P<0.001)and CKMT1A level was positively correlated with PAX8 level(r=0.526,P<0.001).CRABP2 was positively correlated with PAX8(r=0.493,P<0.001).There were significant differences in serum CKMT1A,CRABP2 and PAX8 levels among endometrial cancer patients with different myometrial invasion,differentiation degree and lymph node metastasis(P<0.05).The serum levels of CKMT1A,CRABP2 and PAX8 increased with the decrease of differentiation degree and lymph node metastasis(P<0.05).Twelve patients were included in the recurrence group and 95 patients were included in the non-recurrence group.The serum levels of CKMT1A,CRABP2 and PAX8 in the recurrence group were higher than those in the non-recurrence group,and the differences were statistically significant(P<0.05).The results of ROC curve analysis showed that the area under the curve of CKMT1A,CRABP2 and PAX8 for predicting the recurrence of endometrial cancer were 0.886,0.850 and 0.811 respectively,which were lower than 0.978 of the combined detection of the three(Z combination-CKMT1A=2.318,P=0.020,Z combination-CRABP2=2.030,P=0.042,Z combination-PAX8=2.955,P=0.003).Conclusion CKMT1A,CRABP2 and PAX8 are highly expressed in the serum of patients with endometrial cancer,and their expressions are related to the clinicopathological parameters such as pathological type,differentiation degree,lymph node metastasis and prognosis,which can be used as biological indicators to evaluate the prognosis of patients with endometrial cancer.
作者
董仙萍
于倩
刘英杰
厉昕妤
郭佳
杨小杰
DONG Xianping;YU Qian;LIU Yingjie;LI Xinyu;GUO Jia;YANG Xiaojie(Department of Obstetrics and Gynecology,Tangshan Maternal and Child Health Hospital,Tangshan,Hebei 063000,China;Department of Obstetrics and Gynecology,Affiliated Hospital of North China University of Science and Technology,Tangshan,Hebei 063000,China)
出处
《检验医学与临床》
CAS
2024年第20期2970-2974,2979,共6页
Laboratory Medicine and Clinic
基金
河北省医学科学研究课题(20241138)。
关键词
线粒体肌酸激酶1A
细胞视黄酸结合蛋白2
配对盒基因8
子宫内膜癌
临床病理参数
预后
mitochondrial creatine kinase 1A
cell retinoic acid binding protein 2
paired box gene 8
endometrial cancer
clinicopathological parameter
prognosis
