摘要
目的探讨血清外泌体小泛素样修饰子1伪基因3(SUMO1P3)和肺腺癌转移相关转录本1(MALAT1)对三阴性乳腺癌(TNBC)患者术后复发转移的预测价值。方法选取2016年至2020年行手术切除的224例TNBC患者作为研究对象,根据随访期内复发及转移情况分为非复发转移组和复发转移组,收集两组患者的临床病历资料。采用实时荧光定量PCR(qPCR)检测术前血清外泌体SUMO1P3和MALAT1表达,绘制受试者工作特征(ROC)曲线评价SUMO1P3和MALAT1对TNBC术后复发转移的预测价值。采用多因素Logistic回归模型分析影响TNBC术后复发转移的因素。结果共72例患者出现复发或转移。224例TNBC患者SUMO1P3和MALAT1相对表达量分别为3.06±0.68和2.75±0.66,其中复发转移组SUMO1P3和MALAT1相对表达量为3.44±0.67和3.46±0.80,均显著高于非复发转移组的2.88±0.61和2.33±0.48(P<0.001)。ROC曲线结果显示,血清外泌体SUMO1P3和MALAT1联合检测的曲线下面积(AUC)=0.842(95%CI:0.787~0.887),特异度为0.790,灵敏度为0.806,且两指标联合预测的效能优于SUMO1P3和MALAT1单独预测(P<0.05)。多因素Logistic回归模型结果显示,SUMO1P3(OR=4.463,95%CI:2.125~9.372)和MALAT1(OR=9.103,95%CI:4.462~18.573)表达是影响TNBC术后复发转移的独立因素(P<0.05)。结论血清外泌体SUMO1P3和MALAT1与TNBC患者预后密切相关,两指标联合检测对TNBC术后复发或转移具有较高的预测价值。
Objective To investigate the predictive value of serum exosome small ubiquitin-like modifier-1 pseudogene 3(SUMO1P3)and metastasis associated lung adenocarcinoma transcript 1(MALAT1)in postoperative recurrence and metastasis in patients with triple-negative breast cancer(TNBC).Methods A total of 224 patients with TNBC who underwent surgical resection from 2016 to 2020 were selected as the study subjects.According to the recurrence and metastasis during the follow-up period,they were divided into non-recurrence metastasis group and recurrence metastasis group.Clinical medical records of the two groups were collected.Real-time quantitative fluorescent PCR(qPCR)was used to detect the expression of serum exosomes SUMO1P3 and MALAT1 before surgery,and receiver operating characteristic(ROC)curve was drawn to evaluate the predictive value of SUMO1P3 and MALAT1 in postoperative recurrence and metastasis of TNBC.Multivariate Logistic regression model was used to analyze the factors affecting recurrence and metastasis after TNBC.Results A total of 72 patients had recurrence or metastasis.The relative expression levels of SUMO1P3 and MALAT1 in 224 TNBC patients were 3.06±0.68 and 2.75±0.66,respectively,among which the relative expression levels of SUMO1P3 and MALAT1 in the relapsed and metastatic group were 3.44±0.67 and 3.46±0.80,respectively.They were significantly higher than 2.88±0.61(P<0.001)and 2.33±0.48(P<0.001)in the non-recurrent metastasis group.ROC curve results showed that AUC of serum exosome SUMO1P3 and MALAT1 combined detection was 0.842(95%CI:0.787-0.887),the specificity was 0.790,the sensitivity was 0.806,and the combined prediction efficiency of the two indexes was better than that of SUMO1P3 and MALAT1 alone(P<0.05).Multivariate Logistic regression model showed that SUMO1P3(OR=4.463,95%CI:2.125-9.372)and MALAT1(OR=9.103,95%CI:4.462-18.573)expression was an independent factor affecting the recurrence and metastasis after TNBC(P<0.05).Conclusion Serum exosomes SUMO1P3 and MALAT1 are closely related to the prognosis of TNBC patients,and the combined detection of the two indicators has a high predictive value for postoperative recurrence or metastasis of TNBC.
作者
郭向阳
苗立峰
张国琛
GUO Xiangyang;MIAO Lifeng;ZHANG Guochen(Department of Breast Surgery,Shanxi Cancer Hospital,Taiyuan 030013,China)
出处
《临床肿瘤学杂志》
CAS
2024年第6期561-565,共5页
Chinese Clinical Oncology
关键词
三阴性乳腺癌
外泌体
小泛素样修饰子1伪基因3
肺腺癌转移相关转录本1
复发
转移
Triple-negative breast cancer(TNBC)
Exosome
Small ubiquitin-like modifier-1 pseudogene 3(SUMO1P)
Lung adenocarcinoma metastasis-related transcript 1(MALAT1)
Recurrence
Metastasis
