摘要
为了解不同地区青稞材料的遗传多样性,挖掘与农艺性状相关的标记,该研究利用92对SSR标记分析来自甘肃和西藏不同地区的102份青稞材料的遗传多样性和群体遗传结构,并利用Tassel 2.1软件的GLM模型进行SSR标记与农艺性状的关联分析。结果表明,92对标记共扫描鉴定出739个等位基因,基因多样性指数变幅为0.106~0.913,多态性信息含量变幅为0.103~0.907;遗传相似系数变幅为0.680~0.960,聚类分析将这些青稞材料分为2个亚群,具有相同亲本或亲缘关系相近的材料聚在一起;群体遗传结构分析把青稞材料分为5个亚群;鉴定出33个标记与9个农艺性状在P<0.01水平上极显著关联,与千粒重相关的标记有10个,其中标记GBM1238较为稳定,这些标记的表型变异解释率为8.95%~35.02%。综上,该研究大部分供试青稞材料的亲缘关系相近,可以引进不同的青稞遗传资源来丰富遗传背景。
In order to understand the genetic diversity of hulless barley germplasms from different regions and to discover markers associated with agronomic traits.In this study,we analyzed the genetic diversity and population genetic structure of 102 hulless barley germplasms from different regions of Gansu and Xizang using 92 pairs of SSR markers,and the association analysis between SSR markers and agronomic traits was carried out by using the GLM model of Tassel 2.1 software.The results showed that a total of 739 alleles were identified by scanning 92 markers,and the variance of gene diversity index ranged from 0.106 to 0.913,the variance of polymorphism information content ranged from 0.103 to 0.907;the genetic similarity coefficient varied from 0.680 to 0.960,and the clustering analysis divided these hulless barley germplasms into two subgroups,germplasms with the same parentage or similar kinship were clustered together;the population genetic structure analysis divided the hulless barley germplasms into five subgroups;it was revealed that more than 9 agronomic traits of 33 markers were extremely remarkalbe at the P<0.01 level,there were 10 markers associated with thousand grain weight,of which the marker GBM1238 was more stable,and the explained rate of the phenotypic variance of these markers ranged from 8.95%to 35.02%.In conclusion,most of the hulless barley germplasms for the test in this study are similarly related,and different hulless barley genetic resources can be introduced to enrich the genetic background.
作者
张元元
冯举伶
肖婧凤
关宇
龙楚儿
姚立蓉
孟亚雄
司二静
李葆春
马小乐
王化俊
周喜荣
刘梅金
汪军成
ZHANG Yuanyuan;FENG Juling;XIAO Jingfeng;GUAN Yu;LONG Chuer;YAO Lirong;MENG Yaxiong;SI Erjing;LI Baochun;MA Xiaole;WANG Huajun;ZHOU Xirong;LIU Meijin;WANG Juncheng(State Key Laboratory of Aridland Crop Science/Gansu Provincial Key Laboratory of Crop Improvement&Germplasm Enhancement,Lanzhou 730070,China;College of Agronomy,Gansu Agricultural University,Lanzhou 730070,China;College of Life Science and Techology,Gansu Agricultural University,Lanzhou 730070,China;Gannan Tibetan Autonomous Prefecture Agricultural Science Research Institute,Hezuo 747000,Gansu,China)
出处
《浙江农业学报》
CSCD
北大核心
2024年第9期1977-1989,共13页
Acta Agriculturae Zhejiangensis
基金
国家现代农业产业技术体系项目(CARS-05-04B-2)
甘肃省科技厅科技计划-科技特派团专项(22CX8 NA047,22CX8NP248)
甘肃省高等学校产业支撑计划项目(2021CYZC-12)
甘肃农业大学伏羲青年英才计划(Gaufx-03Y06,Gaufx-04Y011)
甘肃农业大学大学生创新创业训练计划(202401042)
甘南州科技计划项目(2022JY1NC001)
甘肃省科技重大专项(17ZD2 NA016)。
关键词
青稞
SSR
农艺性状
遗传多样性
关联分析
hulless barley
SSR
agronomic trait
genetic diversity
association analysis
