摘要
乌鳖返春口服液是一种结合了现代药物与食品特性的产品。本研究采用网络药理学方法,利用TCMSP数据库并结合相关文献查找其活性成分;通过SwissTargetPrediction和PharmMapper数据库预测其活性成分的可能作用靶点,在Genecards、DisGeNET和OMIM数据库检索、筛选活性成分作用的相关靶点;利用STRING数据库构建潜在作用靶点的蛋白相互作用(PPI)网络,筛选出该口服液活性成分作用的关键靶点网络,并对这些关键靶点进行GO功能和KEGG通路富集分析,确定其核心通路,以构建该口服液活性成分的作用网络。结果表明,共获得该口服液活性成分75个,基于活性成分得到384个潜在作用靶点,通过潜在作用靶点PPI网络筛选得到75个关键靶点。GO功能富集到892个条目,KEGG富集到164条通路。综合以上结果,认为该口服液活性成分作用机制可能是通过大黄蒽醌类、黄酮类和植物甾醇类化合物作用于AKT1、TNF和IL6核心靶点,以PI3KAkt、HIF-1、mTOR和FoxO为信号通路来实现。
Wubiefanchun oral liquid is a kind of product which combines the characteristics of modern medicine and food.The active ingredients were found by using TCMSP database and related literature using network pharmacology method.The potential targets of active ingredients were predicted by SwissTargetPrediction and PharmMapper databases,and the related targets were searched and screened in Genecards,DisGeNET and OMIM databases.The protein-protein interaction(PPI)network of the potential target was constructed using STRING database,and the key target network of the active ingredient of the oral liquid was screened out,and the GO function and KEGG pathway enrichment analysis were performed on these key targets to determine their core pathway,so as to construct the action network of the active ingredient of the oral liquid.The results showed that a total of 75 active ingredients were obtained,384 potential targets were obtained based on the active ingredients,and 75 key targets were obtained through potential target PPI network screening.GO was enriched to 892 entries and KEGG was enriched to 164 pathways.Based on the above results,the mechanism of action of the active ingredients of the oral liquid may be realized through the action of rhubarb anthraquinones,flavonoids and phytosterols on the core targets of AKT1,TNF and IL6,with PI3K-Akt,HIF-1,mTOR and FoxO as signaling pathways.
作者
于茜
朱媛媛
周竹琇
左文婷
YU Qian;ZHU Yuanyuan;ZHOU Zhuxiu;ZUO Wenting(Affiliated Hospital of Nanjing University of Traditional Chinese Medicine,Nanjing 210029,China;Jiangsu Provincial Hospital of Traditional Chinese Medicine,Nanjing 210029,China)
出处
《安徽农学通报》
2024年第21期94-98,共5页
Anhui Agricultural Science Bulletin
基金
江苏省中医药管理局面上项目(MS2021017)。
关键词
乌鳖返春口服液
药食同源
活性成分筛选
网络药理学
Wubiefanchun oral liquid
homology of medicine and food
screening of active ingredients
network pharmacology
