摘要
【目的】为明确金线莲共生木霉菌生长与产孢的最佳条件。【方法】以3株自主分离的哈茨木霉A21B-1、拟康宁木霉A21B-2与Trichoderma longifialidicum A21E为材料,应用十字交叉法与血球计数法分析不同温度、pH值、碳源和氮源对其菌丝生长与产孢的影响。【结果】3株木霉菌菌株在30℃时最适合菌丝生长和产孢;pH值均为4时,是3株木霉菌菌丝生长的最佳pH值,3株木霉菌产孢的最适pH值均为4~6;木糖最有利于A21B-1产孢;麦芽糖、果糖和葡萄糖均适宜A21B-2的菌丝生长产孢,葡萄糖和麦芽糖适宜A21E的菌丝生长和产孢;A21B-1适宜产孢的氮源为牛肉膏、蛋白胨和硫酸铵;适宜A21B-2和A21E产孢的氮源为牛肉膏和蛋白胨。【结论】综合考虑成本、操作流程和生产工艺难度等,3株木霉菌生长和产孢的适宜条件是30℃,pH值为4,麦芽糖,蛋白胨。
【Objective】In order to identify the optimal conditions for mycelial growth and sporulation of three selfisolated Trichoderma strains.【Method】The effects of different temperatures,pH,carbon sources,and nitrogen sources on mycelial growth and sporulation of three self-isolated Trichoderma strains:Trichoderma harzii A21B-1,Trichoderma koningiopsis A21B-2,and T.longifialidicum A21E were analyzed by cross method and blood cell counting method.【Result】The results showed that the three strains of Trichoderma were most suitable for mycelial growth and sporulation at 30°C;the optimal pH for mycelial growth of all three strains was 4;the pH for spore production of all three strains was 4-6;xylose was most favorable for sporulation of A21B-1;maltose,fructose,and glucose were suitable for mycelial growth and sporulation of A21B-2;glucose and maltose were suitable for mycelial growth and sporulation of A21E;the nitrogen source suitable for sporulation of A21B-1 was beef extract,peptone,and ammonium sulfate;the nitrogen source suitable for sporulation of A21B-2 and A21E was beef extract and peptone.【Conclusion】Considering the cost,operation process and difficulty of production technology,the felicity conditions for mycelial growth and sporulation of three Trichoderma strains were 30℃,pH 4,maltose and peptone.
作者
李尊文
王培育
颜沛沛
江金兰
杜昀玥
干小丹
麻成龙
叶炜
Li Zunwen;Wang Peiyu;Yan Peipei;Jiang Jinlan;Du Yunyue;Gan Xiaodan;Ma Chenglong;Ye Wei(Sanming Academy of Agricultural Sciences/Fujian Key Laboratory of Crop Genetic Improvement and Innovative Utilization for Mountainous Areas,Sanming,Fujian 365509,China;College of Agriculture,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China)
出处
《东南园艺》
2024年第5期367-377,共11页
Southeast Horticulture
基金
福建省引导性项目(2023N0047)
三明市科技计划项目(23-N-6)。
关键词
木霉菌
培养条件
菌丝生长
产孢量
Trichoderma
Culture conditions
Hyphal growth
Sporulation quantity
