摘要
目的了解7株水产品来源的霍乱弧菌污染情况、血清分型、毒力基因和全基因组的遗传特征。方法对2023年采集自辽宁省餐饮环节和流通环节的鱼类、腹足类、虾类和双壳类等共163份水产样品,进行霍乱弧菌的检测,对分离菌株进行血清分型、毒力基因检测和全基因组测序分析。结果163份样品中共检出7株霍乱弧菌,检出率为4.3%,其中6株菌为非O1/O139群,1株为O1群稻叶血清型,所有分离株均不携带ctxAB基因,7株菌的基因组序列长度为3853887~4164351 bp,GC含量为47.07%~47.76%,编码序列数量为3370~3732个,串联重复序列数量为55~73个,基因岛的数量为4~11个。7个分离株包含588~603个毒力相关基因,携带耐药基因的数量为263~286个。7株霍乱弧菌共有2863个共有的基因家族,O1群稻叶血清型菌株含有104个特有基因家族,同源单拷贝基因构建进化树共分为两个簇,其中第一簇中包含2个分离株,且高度同源,第二簇中包含其他5株菌株。结论7个霍乱分离株携带多种毒力相关基因和耐药基因,O1群稻叶血清型分离株各基因序列来源复杂,决定了其对环境的适应性。通过本研究了解霍乱弧菌的基因组特征,为食品安全风险评估、基因组组分和功能研究提供数据支持。
Objective To understand the contamination,serotyping,virulence genes and genetic characteristics of the whole genome of 7 strains of Vibrio cholerae from aquatic products.Methods A total of 163 aquatic samples of fish,gastropods,shrimps and bivalves were collected from catering and circulation links in Liaoning Province in 2023 for the detection of Vibrio cholerae.Serotyping,virulence gene detection and whole genome sequencing analysis were performed on the isolated strains.Results A total of 7 strains of Vibrio cholerae were detected in 163 samples,with a detection rate of 4.3%.Among them,6 strains were non-O1/O139 group and 1 strain was O1 group rice leaf serotype.All isolates did not carry the ctxAB gene.The genome sequence length of 7 strains was 3853887-4164351 bp,the GC content was 47.07%-47.76%,the number of coding sequences was 3370-3732,the number of tandem repeat sequences was 55-73,and the number of gene islands was 4-11.The 7 isolates contained 588-603 virulence-related genes,and the number of drug resistance genes was 263-286.There were 2863 common gene families in the 7 strains of Vibrio cholerae.There were 104 unique gene families in the O1 rice leaf serotype strain.The phylogenetic tree constructed by homologous single-copy genes was divided into 2 clusters.The first cluster contained 2 isolates and was highly homologous,and the second cluster contained 5 other strains.Conclusion The 7 cholera isolates carry a variety of virulence-related genes and drug resistance genes.The gene sequences of O1 rice leaf serotype isolates are complex,which determines their adaptability to the environment.This study aims to understand the genomic characteristics of Vibrio cholerae and provide data support for food safety risk assessment,genomic composition and functional studies.
作者
王伟杰
刘海霞
岳航羽
赵韬然
张眉眉
WANG Wei-Jie;LIU Hai-Xia;YUE Hang-Yu;ZHAO Tao-Ran;ZHANG Mei-Mei(Liaoning Provincial Center for Disease Prevention and Control,Shenyang 110172,China)
出处
《食品安全质量检测学报》
CAS
2024年第19期314-320,共7页
Journal of Food Safety and Quality
关键词
霍乱弧菌
毒力基因
血清型
全基因组测序
Vibrio cholerae
virulence genes
serotype
whole genome sequencing
