摘要
应用气相色谱-质谱联用(gas chromatography-mass spectrometer,GC-MS)代谢组学方法考察一贯煎水提物对小鼠抗辐射作用并探索其相关作用通路及作用机制。将健康SPF级雄性昆明小鼠随机分为空白组、模型组、阳性药组以及一贯煎高、中、低剂量组,每组10只。小鼠预防性给药6 d后,采用5 Gy的^(60)Coγ射线照射建立辐射小鼠模型。辐射损伤后观测14 d内小鼠体质量以及外周血变化,于辐射损伤后第3天采集小鼠呼出气,于辐射损伤后第14天采集小鼠血清,应用GC-MS技术分析各组小鼠呼出气和血清,寻找并分析一贯煎抗辐射作用后小鼠的呼出气和血清中差异性代谢物及含量变化。呼出气代谢组学分析结果提示小鼠呼出气中有15种差异性代谢物与辐射损伤有关,其中包括己烷、乙酸乙酯、己醛、乙酸、乙苯、邻二甲苯、1-己醇、苯乙烯、癸烷、辛醛、均三甲苯、D-柠檬烯、苯乙酮、萘和壬醛;血清代谢组学分析结果显示,小鼠血清中有26种差异性代谢物与辐射损伤有关,其中包括乙二醇、乳酸、L-丙氨酸、3-羟基丁酸、L-缬氨酸、甘油、丝氨酸、L-苏氨酸等,通过构建一贯煎抗辐射作用的相关代谢网络,显示有3条代谢通路与一贯煎抗辐射作用相关,其中包括亚油酸代谢,苯丙氨酸、酪氨酸和色氨酸生物合成以及苯丙氨酸代谢。提示一贯煎对辐射损伤小鼠有一定防治作用,该实验对辐射损伤的机制进行了初步探索,为一贯煎防治辐射的作用机制研究奠定了基础。
The gas chromatography-mass spectrometer(GC-MS)metabolomics method was used to investigate the anti-radiation effect of Yiguan Decoction water extract on mice and explore its related pathways and mechanisms of action.Healthy SPF male mice from Kunming were randomly assigned to six groups:blank group,model group,positive drug group,and high-dose,medium-dose,and low-dose groups of Yiguan Decoction,with ten mice in each group.Mice were prophylactically dosed for six days,and ^(60)Coγray with 5 Gy was used to establish a radiation mouse model.Changes in mouse body weight and peripheral blood within 14 days after radiation injury were observed,and exhaled breath from mice on the third day after radiation injury was collected.Mouse serum on the 14th day after radiation injury was collected,and GC-MS technology was applied to analyze the exhaled breath and serum of each group of mice,explore the differential metabolites and changes in content in exhaled breath and serum of mice under the anti-radiation effect of Yiguan Decoction.The metabolomics analysis of exhaled breath suggested that there were 15 differential metabolites in the exhaled breath of mice that were related to radiation injury,including hexane,ethyl acetate,hexanal,acetic acid,ethylbenzene,O-xylene,1-hexanol,styrene,decane,octanal,mesitylene,D-limonene,acetophenone,nonanal,and naphthalene.The results of serum metabolomics analysis showed that 26 differential metabolites in mouse serum were related to radiation injury,including glycol,lactic acid,L-alanine,3-hydroxybutyric acid,L-valine,glycerol,serine,and L-threonine.By constructing a metabolic network pathway related to the anti-radiation effect of Yiguan Decoction,three metabolic pathways were found to be associated with the anti-radiation effect of Yiguan Decoction,including linoleic acid metabolism,biosynthesis of phenylalanine,tyrosine,and tryptophan,and phenylalanine metabolism.The results indicated that Yiguan Decoction had a certain preventive and therapeutic effect on radiation injury in mice,and the mechanism of radiation injury was preliminarily explored,laying a foundation for the research on the mechanism of Yiguan Decoction against radiation.
作者
徐瑞平
董明浩
肖炳坤
李志恒
黄荣清
XU Rui-ping;DONG Ming-hao;XIAO Bing-kun;LI Zhi-heng;HUANG Rong-qing(School of Traditional Chinese Medicine,Guangdong Pharmaceutical University,Guangzhou 511436,China;Institute of Radiation Medicine,Academy of Military Medical Sciences,Academy of Military Sciences,Beijing 100850,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2024年第19期5335-5342,共8页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(21375147)。
