摘要
为探究健康樟子松天然林和不同地区樟子松枯梢病林下土壤真菌多样性差异,以红花尔基自然保护区樟子松健康天然林为对照,红花尔基自然保护区樟子松天然林、泰来县和章古台人工林中的感病樟子松为研究对象,采用lllumina Miseq高通量测序技术分析不同樟子松林地土壤真菌群落组成。结果表明,来自3个地区的4组樟子松林下土样中,总共获得5564个真菌扩增子序列变异体(Amplicon Sequence Variant,ASV),其中担子菌门(Basidiomycota)和子囊菌门(Ascomycota)的相对丰度较高;不同地区不同土壤深度的优势属丰度及种类存在差异,健康天然林表层土壤真菌优势属为糙缘腺革菌属(Amphinema),0~10 cm和10~20 cm深土壤真菌优势属为丝膜菌属(Cortinarius);感病樟子松林下土壤中,天然林表层、0~10 cm和10~20 cm深土壤真菌优势属分别为木霉属(Trichoderma)、篮状菌属(Talaromyces)和乳牛肝菌属(Suillus),泰来县人工林表层、0~10 cm和10~20 cm深土壤真菌优势属分别为Delastria属、假裸囊菌属(Pseudogymnoascus)和Sagenomella属,章古台人工林表层土壤真菌优势属为Knufia属,0~10 cm和10~20 cm深土壤真菌优势属均为假裸囊菌属。此外,感病人工林土壤真菌群落中的植物病原菌占比分别为2.63%、5.04%,远低于感病天然林(8.77%)和健康天然林(8.11%)。人工林土壤真菌群落中的外生菌根含量低于天然林。推测人工林由于其树种的单一性导致外生菌根真菌丰富度较低,使得樟子松的抗病性较差。
In order to investigate the differences in soil fungal diversity between healthy Pinus sylvestris var.mongolica natural for⁃ests and different areas of blight disease-susceptible P.sylvestris forests,this study used healthy natural forests of P.sylvestris in Hong⁃huaerji Nature Reserve as the control,susceptible P.sylvestris in natural forests of Honghuaerji Nature Reserve,and in plantation for⁃ests in Tailai County and Zhanggutai as the study objects.The soil fungal community composition was analyzed using lllumina Miseq high-throughput sequencing technology.The results showed that 5564 fungal Amplicon Sequence Variants(ASV)were obtained from the soil samples of 4 groups from three regions,with higher relative abundance of Basidiomycota and Ascomycota.The abundance and species of dominant genera varied in different soil depths in different regions.The dominant genus of soil fungi in the surface layer of healthy natural forests was Amphinema,and the dominant genus of soil fungi in the 0-10 cm and 10-20 cm depths was Cortinarius.In the understorey soil of susceptible P.sylvestris,the dominant genera of soil fungi in the surface layer,0-10 cm and 10-20 cm depth of natural forests were the genera of Trichoderma,Talaromyces and Suillus,and the dominant genera of soil fungi in the surface layer,0-10 cm and 10-20 cm depth of plantation forests in Tailai County were the genera of Delastria,Pseudogymnoascus and Sagenomella,and the dominant genus of soil fungi in the surface layer of Zhanggutai plantation forest was Knufia,while the dominant genus of soil fungi in the 0-10 cm and 10-20 cm depths were Pseudogymnoascus.In addition,the phytopathogenic fungi in the soil fungal commu⁃nity of the susceptible plantation forests were in the percentages of 2.63%and 5.04%,respectively,which were much lower than those of the susceptible natural forests(8.77%)and healthy natural forests(8.11%).The content of ectomycorrhizal in the soil fun⁃gal community of plantation was lower than that of natural forest.It is hypothesised that plantation forests have a lower abundance of exogenous mycorrhizal fungi due to their monoculture,making P.sylvestris less resistant to disease.
作者
刘心葵
武崇高
刘雪峰
刁桂萍
LIU Xinkui;WU Chonggao;LIU Xuefeng;DIAO Guiping(College of Forestry,Northeast Forestry University,Harbin 150040,China)
出处
《森林工程》
北大核心
2024年第6期41-52,共12页
Forest Engineering
基金
中央高校基本科研业务费专项资金项目(2572022DS07)。
关键词
高通量测序
樟子松
土壤微生物
枯梢病
真菌多样性
High-throughput sequencing
Pinus sylvestris var.mongolica
soil microorganisms
blight disease
fungal diversity
