摘要
建立超高效液相色谱-质谱联用法测定猪肉中恩诺沙星、环丙沙星、氧氟沙星、沙拉沙星4种喹诺酮类药物残留的分析方法。试样经EDTA-Mcllvaine缓冲溶液提取,用HLB固相萃取柱净化,氮吹浓缩后用1 mL甲酸水溶液(0.1%)复溶,上机测定。结果表明,4种喹诺酮类药物残留组分在2.5~100.0μg·L^(-1)线性关系良好,方法检出限为1.0~2.5μg·kg^(-1),定量限为3.0~8.0μg·kg^(-1),加标回收率在90.0%~109.2%,相对标准偏差(Relative Standard Deviation,RSD)为1.01%~5.74%,适用于猪肉中4种喹诺酮类药物残留的检测。
Establish an analytical method for the determination of residues of four quinolone drugs,including enrofloxacin,ciprofloxacin,ofloxacin,and sarafloxacin,in pork using ultra-high performance liquid chromatographymass spectrometry.The sample was extracted with EDTA Mcllvaine buffer solution,purified with HLB solid-phase extraction column,concentrated with nitrogen blowing,and then dissolved in 1 mL formic acid aqueous solution(0.1%)for measurement on the machine.The results showed that there was a good linear relationship between the residual components of the four quinolone drugs in the range of 2.5~100.0μg·L^(-1).The detection limit of the method was 1.0~2.5μg·kg^(-1),and the quantification limit was 3.0~8.0μg·kg^(-1).The recovery rate was in the range of 90.0%~109.2%,and the relative standard deviation(RSD)was 1.01%~5.74%.It is suitable for the detection of four quinolone drug residues in pork.
作者
陈美娜
CHEN Meina(Shenzhen Academy of Metrology&Quality Inspection,Shenzhen 518000,China)
出处
《食品安全导刊》
2024年第31期97-99,共3页
China Food Safety Magazine
关键词
超高效液相色谱-质谱联用法
猪肉
喹诺酮
ultra high performance liquid chromatography-mass spectrometry
pork
quinolone
