摘要
SBP家族基因在植物生长发育过程中具有重要作用。为明确茎用莴苣SBP家族基因染色体分布、启动子顺式作用元件、茎膨大期表达特征及其编码蛋白质的理化性质,本研究以拟南芥SBP蛋白氨基酸序列为参考筛选鉴定莴苣SBP家族基因,进行莴苣SBP家族基因的染色体定位、进化树构建、启动子顺式作用元件分析,构建其编码蛋白质的互作网络,并利用转录组测序技术及实时荧光定量PCR分析茎用莴苣茎膨大期SBP家族基因的表达模式。结果表明,在莴苣基因组中共鉴定到27个SBP基因,可分为7个亚族,不均匀分布于8条染色体上。多个SBP转录因子与植物开花相关蛋白LFY、乙烯响应转录因子(TOE2、TOE3、SMZ、SNZ)存在互作。在茎用莴苣茎膨大过程中,13个SBP基因的表达模式存在差异。随着茎用莴苣茎的膨大,LsSBP25的表达量呈增加趋势,而LsSBP10、LsSBP22的表达量逐步降低。本研究结果可为进一步探究莴苣SBP家族基因在莴苣生长发育过程中的作用提供基础。
SBP family genes play important roles in the growth and development process of plants.In order to identify the chromosomal distribution,promoter cis-acting elements,expression characteristics in stem expansion stage and the physicochemical properties of proteins encoded by SBP family genes,the lettuce SBP family genes were screened and identified based on the amino acid sequence of SBP protein in Arabidopsis thaliana.The chromosome localization,evolutionary tree construction and promoter cis-acting element analysis of SBP family genes in lettuce were performed,and the interaction network of proteins encoded by SBP family genes was constructed.The expression patterns of SBP family genes were analyzed by transcriptome sequencing and quantitative real-time PCR.The results showed that there were 27 SBP genes in lettuce,and they could be classified into seven subgroups.The SBP genes were unevenly distributed on eight chromosomes.Some SBP transcription factors could interact with flowering related protein LFY,ethylene response transcription factors(TOE2,TOE3,SMZ,SNZ).The expression patterns of 13 SBP genes were changed in stem expansion stage of stem lettuce.The expression levels of LsSBP25 increased during the process of stem enlargement,while the expression levels of LsSBP10 and LsSBP22 decreased during the process of stem enlargement.The results of this study can provide a basis for further investigation of the role of SBP family genes in the growth and development of lettuce.
作者
张雨
马文娟
丁怡如
柳天义
廉荣齐
黄莹
ZHANG Yu;MA Wenjuan;DING Yiru;LIU Tianyi;LIAN Rongqi;HUANG Ying(College of Agriculture and Forestry,Linyi University,Linyi 276000,China)
出处
《江苏农业学报》
CSCD
北大核心
2024年第10期1923-1932,共10页
Jiangsu Journal of Agricultural Sciences
基金
山东省自然科学基金项目(ZR2020QC156)
国家自然科学基金青年项目(32102405)
大学生创新创业训练计划项目(X202310452399、X202110452278)。
关键词
莴苣
SBP家族转录因子
茎膨大期
表达模式
lettuce
SBP family transcription factor
stem expansion stage
expression patterns
