摘要
本试验旨在研究口服维生素E(VE)对无特定病原体(SPF)鸡抗肾型传染性支气管炎病毒(IBV)感染作用的免疫学机制。选用40只1日龄SPF白来航雏鸡,随机分为2组,每组20只。1~21日龄,试验组口服100 IU/kg VE(溶于10μL豆油中),对照组口服等量不添加VE的豆油。21日龄时停止口服,全部鸡只通过滴鼻点眼途径人工接肾型IBV强毒株0.1 mL/只,试验期24 d。结果表明:1)口服VE对SPF鸡的生长性能均无显著影响(P>0.05)。2)接种IBV后,2组SPF鸡均出现典型病症,在2 dpi(感染后天数)时,试验组的脾脏和法氏囊中病毒载量显著低于对照组(P<0.05)。3)与对照组相比,试验组的肺脏、脾脏和盲肠扁桃体中(2 dpi)髓样分化因子88(MyD88)的mRNA相对表达量显著降低(P<0.05),肺脏和脾脏中(5 dpi)MyD 88的mRNA相对表达量显著升高(P<0.05),盲肠扁桃体中(5 dpi)P65蛋白(P 65)的mRNA相对表达量显著降低(P<0.05)。4)口服VE对人工感染IBV的SPF鸡组织中鸡β防御素基因的mRNA相对表达量呈多种调控作用,无明显的规律。5)与对照组相比,试验组的脾脏中(2 dpi)和盲肠扁桃体中(5 dpi)CD8阳性细胞(CD8^(+))的mRNA相对表达量显著降低(P<0.05)。综上所述,口服VE对SPF鸡生长性能无影响,但可抑制IBV在机体部分组织中的复制,调控免疫相关因子基因在组织中的表达,在鸡体内抗IBV感染作用中起积极作用。
This experiment was conducted to study the immunological mechanism of oral vitamin E(VE)against nephropathogenic infectious bronchitis virus(IBV)infection in specific pathogen free(SPF)chickens.Forty 1-day-old SPF white leghorn chickens were randomly divided into 2 groups with 20 birds per group.During 1 to 21 days of age,chickens in the experimental group were orally administered 100 IU/kg VE(dissolved in 10μL soybean oil),and chickens in the control group were orally administered equivalent soybean oil without vitamin VE.Stop oral administration at 21 days of age,all the chicken were inoculated with 0.1 mL/bird nephropathogenic virulent IBV strain via combined intraocular and intranasal routes.The experiment lasted for 24 days.The results showed as follows:1)oral VE had no significant effect on growth performance of SPF chickens(P>0.05).2)After inoculated IBV,the SPF chickens in 2 groups demonstrated typical clinical symptoms,on 2 dpi(days post infection),the viral load in spleen and bursa of Fabricius of the experimental group was significantly lower than that of the control group(P<0.05).3)Compared with the control group,the mRNA relative expression level of myeloid differentiation factor 88(MyD88)in lungs,spleen and cecal tonsil(2 dpi)of the experimental group was significantly decreased(P<0.05),the mRNA relative expression level of MyD88 in lungs and spleen(5 dpi)was significantly increased(P<0.05),and the mRNA relative expression level of P65 protein(P65)in cecal tonsil(5 dpi)was significantly decreased(P<0.05).4)Oral VE showed multiple regulation on mRNA relative expression levels of avian beta-defensins genes in tissues of SPF chicken infected with IBV,and little regularity was found.5)Compared with the control group,the mRNA relative expression level of CD8 positive cells(CD8^(+))in spleen(2 dpi)and cecal tonsil(5 dpi)of the experimental group was significantly decreased(P<0.05).In summary,oral VE has no significant effect on the growth performance of SPF chickens.In contrast,oral VE can inhibit the IBV replication in some tissue,regulate the gene expression of immune-related factor in tissues,and plays a positive role in the infection effect of chickens against IBV.
作者
王兵
侯雨彤
张莉莉
张晓娜
韩宗玺
李慧昕
马得莹
WANG Bing;HOU Yutong;ZHANG Lili;ZHANG Xiaona;HAN Zongxi;LI Huixin;MA Deying(College of Animal Science and Technology,Northeast Agricultural University,Harbin 150030,China;State Key Laboratory for Animal Disease Control and Prevention,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,China)
出处
《动物营养学报》
CAS
CSCD
北大核心
2024年第11期7467-7480,共14页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
中央引导地方科技发展专项项目(ZY04JD03)
黑龙江省自然科学基金“禽冠状病毒病分子流行病学及防控技术研究团队”(TD2021C001)
国家现代农业产业技术体系(CARS-40)
黑龙省禽类产业技术协同推广创新体系。
