摘要
[目的]建立聚葡萄糖、甜菊糖苷中蛋白酶活力检测方法。[方法]通过单因素试验、正交试验优化蛋白酶活力的检测条件,考察回收率试验方法准确度。[结果]聚葡萄糖、甜菊糖苷中蛋白酶活力最佳检测条件:硼酸缓冲溶液溶解样品,待测样品取样量5 g,反应时间20 min,反应温度40℃,pH值10.5,滤液静置时间20 min。回收率试验结果表明,方法准确度64%~102%,相对标准偏差<1.3%。[结论]曲线相关性、准确度等验证,该方法适用聚葡萄糖、甜菊糖苷中蛋白酶含量检测。
[Objective]The detection method of protease activity in polyglucose and stevioside glycosides was established.[Method]Single factor experiment and orthogonal experiment were used to optimize the assay conditions of protease activity,and the accuracy of the recovery method was investigated.[Result]The optimal conditions for the detection of protease activity in polyglucose and stevioside glycosides were to dissolve samples with boric acid buffer solution,sample size to be tested was 5 g,reaction time was 20 min,reaction temperature was 40℃,pH value was 10.5,and filtrate standing time was 20 min.The experimental results showed that the accuracy of the method was 64%~102%and the relative standard deviation was less than 1.3%.[Conclusion]This method was suitable for the detection of protease content in stevioside polyglucose after comprehensive verification of curve correlation and accuracy.
作者
张琳
于敏
刘彦明
张亚华
陈娟
逯刚
ZHANG Lin;YU Min;LIU Yanming;ZHANG Yahua;CHEN Juan;LU Gang(Inner Mongolia Mengniu High-Tech Dairy,Co.,Ltd.,Hohhot Inner Mongolia 011500;Inner Mongolia Mengniu Dairy(Group)Co.,Ltd.,Hohhot Inner Mongolia 011500)
出处
《中国乳业》
2024年第11期149-156,共8页
China Dairy
基金
国家重点研发计划项目(2022YFD1301005)。
