摘要
通过将部分亚硝化(PN)与厌氧氨氧化(ANAMMOX)组合形成两段法,研究了该工艺在处理污泥消化液过程中氮素的转化规律、脱氮效果、影响因素及污泥消化液对两段法系统中微生物种群的影响.结果表明两段法可对实际的污泥消化液进行有效处理,在进水氨氮浓度为1000 mg·L^(−1),部分亚硝化反应器和厌氧氨氧化反应器的HRT分别为1d时,两段法对总氮平均去除率为83.37%.不调节进水碱度,PN反应器出水NO_(2)^(-)-N/NH_(4)^(+)-N的比值大于1,满足了ANAMMOX反应对基质浓度的要求,为ANAMMOX反应器的后续运行创造了有利条件.ANAMMOX反应中NH_(4)^(+)-N、NO_(2)^(-)-N的消耗量与NO_(3)^(-)-N生成量之间的比例关系为1∶(1.14±0.10)∶(0.18±0.02),低于理论值.PN和ANAMMOX污泥样品中关键微生物分别为Proteobacteria和Planctomycetes(门水平),在处理消化液前其相对丰度分别为65.2%、38.85%,在处理消化液后其相对丰度分别为35.36%和19.05%.实际消化液中的有机物导致其它异养微生物在两个反应器的污泥中增殖,降低了氨氧化菌(AOB)和厌氧氨氧化菌(AnAOB)的相对丰度,但对其种属无明显影响,因此并不影响两段式PN/A工艺的脱氮效率.通过DO+FNA和DO+FA交替控制PN反应器运行,并通过控制ANAMMOX反应器进水NO_(2)^(-)−N/NH_(4)^(+)-N的比值可实现ANAMMOX的稳定运行,从而保证了两段法工艺对污泥水的高效脱氮处理.
By combining partial nitrification(PN)with anaerobic ammonium oxidation(ANAMMOX)to form a two-stage process,The nitrogen conversion rule,nitrogen removal effect,influencing factors and the effect of reject water on microbial population in the two-stage process were investigated.The results showed that the two-stage method could effectively treat the actual reject water,and the average removal of total nitrogen by the two-stage method was 83.37%at an influent ammonia nitrogen concentration of 1000 mg·L^(-1),and an HRT of 1 d for the partial nitrification reactor and the anaerobic ammonia oxidation reactor,respectively.With no adjustment of influent alkalinity,the ratio of NO_(2)^(-)-N/NH_(4)^(+)-N in the effluent of the PN reactor was greater than 1,which met the requirements of the ANAMMOX reaction on substrate concentration and created favorable conditions for the subsequent operation of the ANAMMOX reactor.The proportionality between the consumption of NH_(4)^(+)-N and NO_(2)^(-)-N and the production of NO_(3)^(-)-N in the ANAMMOX reaction was 1:(1.14±0.10):(0.18±0.02),which was lower than the theoretical value.The key microorganisms in PN and ANAMMOX sludge samples were Proteobacteria and Planctomycetes(phylum level),respectively,whose relative abundance was 65.2%and 38.85%before treatment of reject water,and 35.36%and 19.05%after treatment of reject water,respectively.The organic matter in the actual reject water led to the proliferation of other heterotrophic microorganisms in the sludge of the two reactors,reducing the relative abundance of ammonium oxidation bacteria(AOB)and anaerobic ammonium oxidation bacteria(AnAOB),but had no significant effect on their species.Therefore,the nitrogen removal efficiency of the two-stage PN/A process is not affected.The operation of PN reactor was controlled by DO+FNA and DO+FA alternately,and the stable operation of ANAMMOX could be realized by controlling the ratio of NO_(2)^(-)-N/NH_(4)^(+)-N in the influent of ANAMMOX reactor,so as to ensure the efficient denitrification treatment of sludge water by two-stage process.
作者
李霞
李惠娟
王理明
高敏
梅若晨
张典奥
LI Xia;LI Huijuan;WANG Liming;GAO Min;MEI Ruochen;ZHANG Dianao(School of Environmental l and Chemical Engineering,Xi’an Polytechnic University,Xi’an,710600,China)
出处
《环境化学》
CAS
CSCD
北大核心
2024年第11期3921-3934,共14页
Environmental Chemistry
基金
陕西省教育厅专项科研计划项目(21JK0663,20JK0649)
国家自然科学基金(52100198)
陕西省重点研发计划项目(2019ZDLSF06-05).
关键词
污泥消化液
部分亚硝化
厌氧氨氧化
微生物种群
高通量测序
reject water
partial nitrification(PN)
anaerobic ammonium oxidation(ANAMMOX)
microbial communities
high-throughput sequencing
