摘要
目的通过网络药理学探讨柴胡加龙骨牡蛎汤(Chaihu Longgu Muli Decoction,CLMD)治疗冠状动脉粥样硬化性心脏病(简称“冠心病”)合并焦虑抑郁的机制。方法运用中药系统药理学数据库与分析平台(TCMSP)获得CLMD的药物靶点,GeneCards与在线人类孟德尔遗传(OMIM)数据库获取冠心病、焦虑症和抑郁症的疾病靶点,应用Cytoscape 3.9.1构建“药物-成分-疾病-靶点”网络,基因本体论(GO)与京都基因与基因组百科全书(KEGG)富集分析生物学过程及主要通路。结果CLMD中槲皮素、山柰酚、β-谷甾醇、豆甾醇、汉黄芩素为治疗冠心病合并焦虑抑郁的关键成分,涉及白蛋白(ALB)、丝氨酸/苏氨酸激酶1(AKT1)、白细胞介素-6(IL-6)、肿瘤蛋白P53(TP53)、肿瘤坏死因子(TNF)等40个靶点,GO和KEGG分析结果显示其作用机制与高级糖基化终末产物-受体(AGE-RAGE)、流体剪切应力与动脉粥样硬化、C型凝集素受体、磷脂酰肌醇3激酶(PI3K)-Akt、FoxO等通路有关。结论此研究证实了CLMD治疗冠心病合并焦虑抑郁的多成分、多靶点、多途径机制,为双心疾病的治疗提供了理论依据。
Objective To explore the mechanism of Chaihu Longgu Muli decoction(CLMD)in the treatment of coronary heart disease combined with anxiety and depression through network pharmacology.Methods Using databases such as TCMSP to obtain drug targets for CLMD,GeneCards and OMIM databases to obtain disease targets.Application of Cytoscape 3.9.1 to build a“drugcomponent-disease-target”network,GO and KEGG enrichment to analyze biological processes and major pathways.Results Quercetin,kaempferol,β-sitosterol,stigmasterol and baicalein in CLMD are key components,involving 40 targets including ALB,AKT1,IL-6,TP53 and TNF.GO and KEGG analyses showed that the mechanism of action was related to AGE-RAGE,fluid shear stress and atherosclerosis,C-type lectin receptor,PI3K-Akt,FoxO and other pathways.Conclusion This study confirms the multi-component,multi-target and multi-pathway mechanism of CLMD in the treatment of coronary heart disease combined with anxiety and depressive states,providing a theoretical basis for the treatment of double heart disease.
作者
王馨慧
王苏童
王永成
李晓
WANG Xinhui;WANG Sutong;WANG Yongcheng;LI Xiao(College of Traditional Chinese Medicine,Shandong University of Traditional Chinese Medicine,Shandong Province,Jinan 250014,China;College of Acupuncture and Tuina Science,Shandong University of Traditional Chinese Medicine,Shandong Province,Jinan 250014,China;Department of Cardiology,Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Shandong Province,Jinan 250014,China)
出处
《中国中医药现代远程教育》
2025年第2期147-150,共4页
Chinese Medicine Modern Distance Education of China
基金
中国博士后科学基金面上项目【No.2021M702043】
山东省自然科学基金青年项目【No.ZR2020QH307】。
关键词
胸痹心痛
郁证
冠状动脉粥样硬化性心脏病
焦虑
抑郁
柴胡加龙骨牡蛎汤
网络药理学
precordial pain with chest discomfort
depression syndrome
coronary heart disease
anxiety
depression Chaihu Longgu Muli decoction
network pharmacology
