摘要
采用索氏提取法制备花椒(Zanthoxylum bungeanum Maxim.)精油,利用菌丝生长速率法评价花椒精油对尖镰刀菌(Fusarium oxysporum Schltdl)、禾谷镰孢菌(Fusarium graminearum Schwabe)、盘长孢状刺盘孢(Colletotrichum gloeosporioides Penz.&Sacc.)和茄链格孢菌(Alternaria solani)菌丝生长的抑制活性,采用气相色谱-质谱联用仪(GC-MS)探究花椒精油的物质组成。结果表明,花椒精油对禾谷镰孢菌、尖镰刀菌、盘长孢状刺盘孢和茄链格孢菌均表现出一定的抗菌活性,半数效应浓度(EC_(50))分别为1.552、2.243、6.064、7.078 mg/mL。GC-MS分析显示,花椒精油中的主要成分为萜类化合物,包括乙酸芳樟酯、芳樟醇、D-柠檬烯和桉叶油醇等。
Zanthoxylum bungeanum essential oil was extracted using the Soxhlet method.The effects of Zanthoxylum bungeanum es⁃sential oil on Fusarium oxysporum,Fusarium graminearum,Colletotrichum gloeosporioides and Alternaria solani were evaluated by the mycelial growth rate method.The composition of Zanthoxylum bungeanum essential oil was analyzed using gas chromatography-mass spectrometry(GC-MS).The results showed that Zanthoxylum bungeanum essential oil exhibited antifungal activity Fusarium grami⁃niearum,Fusarium oxysporum,Colletotrichum gloeosporioides and Alternaria solani,with median effect concentration(EC_(50))of 1.552,2.243,6.064,7.078 mg/mL,respectively.GC-MS analysis revealed that the principal constituents of Zanthoxylum zanthoxy⁃lum essential oil were terpenoids including linalyl acetate,linalool,D-limonene and eucalyptol.
作者
李承钊
刘玉杰
王伟峰
孟宪华
曹永红
苏群
王君
杨军丽
LI Cheng-zhao;LIU Yu-jie;WANG Wei-feng;MENG Xian-hua;CAO Yong-hong;SU Qun;WANG Jun;YANG Jun-li(Yantai Zhongke Research Institute of Advanced Materials and Green Chemical Engineering,Yantai 264000,Shandong,China;Shandong Laboratory of Advanced Materials and Green Manufacturing at Yantai,Yantai 264000,Shandong,China;Lanzhou Institute of Chemical Physics,Chinese Academy of Sciences/Key Laboratory of Chemistry of Northwestern Plant Resources/Key Laboratory for Natural Medicine of Gansu Province,Lanzhou 730000,China;Zanthoxylum Bungeanum Research Institute,Longnan Economic Forest Research Institute,Longnan 746000,Gansu,China;Baoyuan Bio-Agri Science&Technology(Shandong)Co.,Ltd.,Yantai 264000,Shandong,China)
出处
《湖北农业科学》
2024年第12期62-66,共5页
Hubei Agricultural Sciences
基金
国家自然科学基金项目(32270419)
山东省自然科学基金资助项目(ZR2021MB089,ZR2023MC040)
烟台先进材料与绿色制造山东省实验室项目(E1R03SXM04)
烟台开发区科技领军项目(2022RC005)
陇南市科技计划项目(2022-S˙QKJ-03)。
