摘要
目的:探究藏药九味防瘟散体内外抗菌抗炎作用.方法:首先,采用散香法和最小抑菌浓度测定九味防瘟散的体外抗菌作用.建立细菌感染小鼠模型,以小鼠存活率和组织菌载量检测体内抗菌作用.随后,以脓毒症小鼠模型来评价九味防瘟散抗感染性炎症的效果.建立LPS诱导的脓毒症模型和CLP脓毒症模型,检测小鼠存活率、肺组织损伤、炎症因子的mRNA和蛋白水平.结果:九味防瘟散有一定抗菌作用,其中体外对肺炎克雷伯菌抑制效果明显,体内能提高细菌感染小鼠的存活率,降低肺炎克雷伯菌感染小鼠的组织菌载量.九味防瘟散能明显抑制感染引起的炎症,提高脓毒症小鼠存活率、减轻肺组织损伤、降低炎症因子产生.结论:九味防瘟散具有显著的抗菌及抗感染作用.
Objective To investigate the antibacterial and anti-inflammatory properties of the Tibetan medicine Jiuwei Fangwen San,both in vitro and in vivo.Methods Initially,the fragrance dispersion method and minimum inhibitory concentration(MIC)determination were employed to assess its antibacterial properties in vitro.A mouse model about bacterial infection was established to evaluate the antibacterial effects using survival rate and tissue bacterial load as indicators in vivo.Subsequently,LPS-and CLP-induced sepsis models were established to evaluate the anti-inflammatory efficacy of Jiuwei Fangwen San using survival rates,lung tissue damage,mRNA and protein levels of inflammatory factors as indicators.Results Jiuwei Fangwen San exerted notable antibacterial activity,particularly against Klebsiella pneumoniae in vitro.It improved the survival rates of mice infected with bacteria and reduced the tissue bacterial load in mice infected with Klebsiella pneumoniae.Furthermore,Jiuwei Fangwen San significantly mitigated inflammation induced by infection,evidenced by increased survival rates in septic mice,alleviated lung tissue damage,and decreased the production of inflammatory factors.Conclusions Jiuwei Fangwen San possessed significant antibacterial and anti-inflammatory properties.This study provided experimental evidence for the clinical application of Jiuwei Fangwen San.
作者
朱文静
周雁南
李昊
罗珍
次仁多吉
更桑
赵洋
窦洁
ZHU Wenjing;ZHOU Yannan;LI Hao;LUO Zhen;CIRenduoji;GENGSang;ZHAO Yang;DOU Jie(China Pharmaceutical University,Nanjing 210009,China;Xizang Tibetan Medical University,Lhasa 850000,China)
出处
《西北民族大学学报（自然科学版）》
2024年第4期85-93,共9页
Journal of Northwest Minzu University(Natural Science)
基金
西藏藏医药大学校级科研项目(2024ZRZD03)。
关键词
藏药
九味防瘟散
抗菌
抗炎
脓毒症
Tibetan medicine
Jiuwei Fangwen San
Antibacterial
Anti inflammation
Sepsis
