摘要
为系统了解石膏的物质基础及其影响因素,采用题示方法同时测定石膏中51种无机元素(常量元素、微量元素、稀土元素、重金属及有害元素)的含量。取0.200 0 g石膏粉末,加入5 mL硝酸,密封,混匀,于220℃微波消解30 min,消解液用水定容至50 mL(原液)。分取1 mL,用水稀释至50 mL(稀释液)。样品稀释液用于元素Ca、S的测定,样品原液用于其余49种元素的测定。采用电感耦合等离子体原子发射光谱法测定元素S、P、Si,电感耦合等离子体质谱法联合内标法测定其余48种元素,IBM SPSS Statistics 26.0软件对含量较高的16种元素进行相关性分析,Simca软件的正交偏最小二乘-判别分析(OPLS-DA)识别引起产地差异的元素,并对石膏中伴生元素的差异以及石膏无机元素与药效及安全性关系进行了探讨。21批样品分析结果显示:石膏中Ca、S含量最高,与理论值基本一致且在不同产地差异不显著(p>0.05);重金属及有害元素Pb、As、Hg含量较低,可能源于石膏采矿、加工过程;Mg、Na、Mn、Fe、Cu、Ti等文献推测具有清热药效协同作用的元素含量与体内经其他途径获得的含量相比基本可以忽略,且在不同产地石膏中含量差异极大,可能不是影响石膏清热作用的关键元素。相关性分析结果显示,39对元素显著正相关(p<0.01),18对元素正相关(p<0.05),可能为石膏成矿或采挖过程的伴生或共生元素。OPLS-DA分析结果显示,Cu、Si可能是引起内蒙古产地石膏与山东、山西产地石膏差异的元素,Na、P可能是引起山西产地石膏与山东产地石膏差异的元素。
To systematically understand the material basis and influencing factors of Gypsum Fibrosum,the title method was used to simultaneously determine the contents of 51 inorganic elements(constant elements,trace elements,rare earth elements,heavy metals and harmful elements)in Gypsum Fibrosum.The 0.2000 g of Gypsum Fibrosum powder was taken,and 5 mL of nitric acid was added.After sealing and mixing well,the mixture was digested by microwave at 220℃for 30 min.The digestion solution was diluted to 50 mL by water(stock solution),and an aliquot(1 mL)was taken and diluted to 50 mL by water(dilution solution).Sample dilution solution and sample stock solution were used for the determination of the Ca,S elements and remaining 49 elements,respectively.S,P and Si elements were determined by inductively coupled plasma atomic emission spectrometry,and the remaining 48 elements were determined by inductively coupled plasma mass spectrometry with internal standard method.IBM SPSS Statistics 26.0 software was used to perform correlation analysis on the 16 elements with high content.Orthogonal partial least squares discriminant analysis(OPLS-DA)in the Simca software was used to identify elements that caused origin differences.The differences in associated elements in Gypsum Fibrosum and the relationship between Gypsum Fibrosum inorganic elements and drug efficacy and safety were discussed.As found by analytical results of 21 batches of samples,the Ca and S contents in Gypsum Fibrosum were the highest,consisting with the theoretical values,and showing no significant difference in different origins(p>0.05).The contents of heavy metals and harmful elements such as Pb,As,and Hg were relatively low,which might originate from Gypsum Fibrosum mining and processing processes.Contents of elements such as Mg,Na,Mn,Fe,Cu,and Ti that might have synergistic antipyretic pharmacological effects described in references could be basically ignored compared to the content obtained through other pathways in the body,which varied greatly in Gypsum Fibrosum from different origins,indicating these elements might not be the key elements affecting the antipyretic effect of Gypsum Fibrosum.As found by correlation analysis results,39 pairs of elements were significantly positively correlated(p<0.01),and 18 pairs of elements were positively correlated(p<0.05),which might be associated or symbiotic elements in Gypsum Fibrosum mineralization or mining processes.As found by OPLS-DA analytical results,Cu and Si might be elements that caused differences of Gypsum Fibrosum between Inner Mongolia and Shandong and Shanxi,while Na and P might be elements that caused differences of Gypsum Fibrosum between Shanxi and Shandong.
作者
丁贝贝
李丽敏
曹帅
彭韦楠
周如洁
胡青
毛秀红
季申
DING Beibei;LI Limin;CAO Shuai;PENG Weinan;ZHOU Rujie;HU Qing;MAO Xiuhong;JI Shen(School of Pharmacy,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;National Medical Products Administration Key Laboratory for Quality Control of Traditional Chinese Medicine,Shanghai Institute for Food and Drug Control,Shanghai 201203,China)
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2024年第12期1231-1239,共9页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
基金
上海市中药和保健食品品质与安全检测专业技术服务平台(21DZ2290200)
矿物药配方颗粒质量控制关键技术研究与标准制订(21DZ2202900)。
关键词
石膏
无机元素
电感耦合等离子体质谱法
电感耦合等离子体原子发射光谱法
产地判别
Gypsum Fibrosum
inorganic element
inductively coupled plasma mass spectrometry
inductively coupled plasma atomic emission spectrometry
origin distinguishment
