摘要
本文合成了具有优异类氧化物酶催化活性的Mn_(3)O_(4)纳米酶,对其形貌、结构和组成进行了表征。3,3′,5,5′-四甲基联苯胺(TMB)显色体系验证了Mn_(3)O_(4)纳米酶的类氧化物酶活性,Mn_(3)O_(4)对TMB具有较高的亲和力和催化效率,米氏常数(K_(m))为0.03146 mmol/L,最大反应速率(V_(max))为5.362×10^(-7) mol/(L·s)。依据全氟辛烷磺酸(PFOS)对显色体系的抑制作用,建立了基于Mn_(3)O_(4)纳米酶的比色传感器用于PFOS的检测。实验显示,PFOS浓度在12~50μmol/L范围内与吸光度呈线性关系,检出限为3.595μmol/L。在实际水样中添加10、30、50μmol/L的PFOS,其加标回收率为99.2%~104.5%,相对标准偏差为1.37%~7.17%。结果表明,该方法具有良好的抗干扰能力、重复性和稳定性,为检测环境样品中的PFOS提供了一种新方法。
Perfluorinated compounds(PFCs)one of the persistent organic environmental pollutants(POPs),are difficult to degrade in the natural environment,which accumulate in the environment and human body,and have systemic multi-organ toxicity,which is.In this paper,we synthesized Mn_(3)O_(4) nanozyme with excellent oxidase(OXD)catalytic activity,characterized their morphology,structure and composition,and verified their OXD-like activity by TMB color system.Mn_(3)O_(4) has high affinity and catalytic efficiency for TMB,with a K m value of 0.03146 mmol/L and a V max value of 5.362×10^(-7) mol/(L·s).Based on the inhibition effect of perfluorooctane sulfonate(PFOS)on the color system,a colorimetric sensor based on Mn_(3)O_(4) nanozyme was established for the detection of PFOS.The concentration of PFOS showed a linear relationship with the absorbance in the range of 12-50μmol/L with a limit of detection of 3.595μmol/L.The method has good anti-interference ability,repeatability and stability.And satisfactory recoveries were obtained when applied for the determination of PFOS in real water samples,with the recoveries ranging from 99.2% to 104.5% and the relative standard deviations(RSDs)ranging from 1.37% to 7.17%.The experimental results demonstrated that the colorimetric sensing based on Mn_(3)O_(4) nanozyme provided a new method for the detection of PFOS in environmental samples.
作者
张晓颖
成瑶
崔宏
ZHANG Xiaoying;CHENG Yao;CUI Hong(School of Public Health,Shanxi Medical University,Taiyuan 030001)
出处
《分析科学学报》
CAS
CSCD
北大核心
2024年第6期634-640,共7页
Journal of Analytical Science
基金
国家自然科学基金(81803286)
山西省自然科学基金(202203021211233)
山西省高等学校科技创新项目(2020L0190)。
