摘要
目的 :研究血栓素合酶 (TXS)和前列环素合酶 (PGI2 -S)基因表达变化与慢性低氧高二氧化碳性肺动脉高压的关系。方法 :将SD大鼠分为正常对照组 ,4周低O2 高CO2 组。观察两组大鼠肺动脉平均压 (mPAP)、右心指数、肺细小动脉显微及超微结构改变 ,肺细小动脉血栓素合酶和前列环素合酶的基因表达变化。结果 :低O2 高CO2 组mPAP、右心指数、血浆及肺匀浆TXB2 和 6 -keto -PGF1 α 含量、肺动脉管壁TXSmRNA表达皆显著高于正常对照组 (P <0 0 1 ) ,而肺动脉管壁PGI2 -SmRNA表达无明显变化 ;低O2 高CO2 组内弹力板扭曲 ,中膜平滑肌细胞增生 ,管腔明显狭窄。结论 :血栓素合酶和前列环素合酶基因表达变化所导致的TXA2 和PGI2
AIM: To study the effect of chronic hypoxic hypercapnia on gene expression of thromboxane synthase and prostacyclin synthase in pulmonary arterioles. METHODS: Sprague-Dawley rats were randomly divided into two groups: control group and hypoxic hypercapnic group. TXS mRNA and PGI 2-S mRNA were observed in pulmonary arterioles by in situ hybridization. RESULTS:mPAP, weight ratio of right ventricle (RV) to left ventricle plus septum(LV+S), contents of TXB 2 and 6-keto-PGF1 α in plasma and lung and TXS mRNA in pulmonary arterioles were much higher in rats of hypoxic hypercapnic group than those of control group. Differences of PGI 2-S mRNA in pulmonary arterioles were not significant in two groups. Light microscopy showed hypertrophy of vessel smooth muscle cells and vessel cavity straitness were found in hypoxic hypercapnic group. CONCLUSION: Changes of gene expressions of thromboxane synthase and prostacyclin synthase and imbalance of TXA 2/PGI 2 may play an important role in hypoxic hypercapnic pulmonary hypertension.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2002年第12期1497-1501,共5页
Chinese Journal of Pathophysiology
基金
浙江省自然科学基金资助项目 (No.30 2 762 )
关键词
血栓烷合酶
前列环素合酶
低氧
高碳酸血
肺性高血压
基因
Thromboxane synthase
Prostacyclin synthase
Anoxia
Hypercapnia
Hypertension, pulmonary
Genes
