摘要
植物蔗糖转运蛋白(sucrose transporter,SUT)主要介导蔗糖质外体运输,在植物生长发育过程中发挥重要作用。为探讨马铃薯蔗糖转运蛋白基因StSUT2的表达模式,通过TOPO定向克隆了StSUT2基因上游1700 bp启动子序列,对其进行生物信息学分析;并利用荧光定量PCR技术检测了StSUT2基因在不同组织器官、不同光照时间下的表达特性。结果表明,马铃薯StSUT2启动子区域内存在多个光响应元件以及脱落酸等激素和胁迫响应元件;StSUT2基因于光照4 h后在花和老叶中表达量较高;光照16 h后在花中表达量最高;此外,StSUT2在雄蕊和雌蕊中的表达量显著高于全花。综上,StSUT2在花器官尤其在花蕊组织的发育中发挥重要作用。研究结果可为深入研究马铃薯StSUT2的生物学功能提供参考。
Plant sucrose transporter(SUT)mainly mediates the transport of sucrose extracellular vesicles and plays an important role in plant growth and development.To investigate the expression characteristics of sucrose transporter gene StSUT2,cloned the upstream 1700 bp promoter sequence of the StSUT2 gene using TOPO and performed bioinformatics analysis on it;and the expression pattern of StSUT2 gene in different organs and tissues after different light time was analyzed by qRT-PCR technology.The results showed that there are several photo-responsive elements,and other response elements such as abscisic acid,salicylic acid,auxin,anaerobic induction,defense and stress response in StSUT2 promoter region.StSUT2 has the highest expression level in flowers and old leaves after 4 h lighting,and in flowers after 16 h lighting,while StSUT2 has a certain expression level in young leave,mature leave,stem,root and stolon.Except for the old leave,StSUT2 expression level in various organs and tissues is not significantly affected by the light time.Furthermore,StSUT2 expression level was significantly higher in stamen and pistil than in whole flower,which suggests that StSUT2 plays an important role in the development of flower organs,especially in stamen tissue.These results provide a reference for intensive investigation of the biological function of StSUT2.
作者
巩慧玲
杨雅倩
梁金永
田泽
冯再平
鲍婧婷
GONG Huiling;YANG Yaqian;LIANG Jinyong;TIAN Ze;FENG Zaiping;BAO Jingting(School of Life Science and Engineering,Lanzhou University of Technology,Lanzhou 730050,Gansu,China)
出处
《中国瓜菜》
北大核心
2025年第1期25-30,共6页
China Cucurbits And Vegetables
基金
国家自然科学基金(31860397)
甘肃省自然科学基金(重点项目)(22JR5RA228)
甘肃省科技计划(技术创新引导计划-科技专员专项)(23CXGA0061)。
