摘要
目的 探讨胃癌患者术前血清GINS复合物4 (GINS4)、PD-1水平与临床病理特征及预后的关系。方法 选取我院2016年8月至2018年8月治疗的95例胃癌患者,并根据患者生存情况分为生存组和死亡组;选取同期95例健康体检者作为对照组。采用酶联免疫吸附试验(ELISA)检测术前血清PD-1水平。采用实时定量PCR检测术前血清GINS4 m RNA水平;采用Kaplan-Meier法分析胃癌患者术前血清GINS4 mRNA、PD-1水平与5年生存率的关系;采用Cox回归分析胃癌预后的影响因素。结果 胃癌组血清GINS4 mRNA、PD-1水平显著高于对照组(P <0.05)。组织低分化、TNM分期Ⅲ~Ⅳ期、有淋巴结转移胃癌患者血清GINS4mRNA、PD-1水平显著高于组织中/高分化、TNM分期Ⅰ~Ⅱ期和无淋巴结转移患者(P <0.05)。死亡组血清GINS4 mRNA、PD-1水平及TNM分期Ⅲ~Ⅳ期、有淋巴结转移患者比例显著高于生存组(P <0.05)。Kaplan-Meier法分析结果显示,胃癌患者血清中GINS4 mRNA高表达、PD-1高表达患者5年生存率低于GINS4 mRNA低表达、PD-1低表达患者(P <0.05)。GINS4 mRNA、PD-1水平是胃癌患者死亡的独立危险因素(P <0.05)。结论 胃癌患者血清GINS4 m RNA、PD-1水平升高,二者与组织分化程度、TNM分期、淋巴结转移及预后密切相关。
Objective To analyze the expression levels of serum GINS complex 4(GINS4) and PD-1 in patients with gastric cancer before surgery and to explore the relationship between these two factors and the clinicopathological characteristics and prognosis of these patients.Methods A total of 95 patients with gastric cancer treated at the First People's Hospital of Nanyang between August 2016 and August 2018 were included in this study.The patients were followed-up for 5 years and divided into survival and death groups based on their survival at the end of the follow-up.A total of 95 healthy individuals in the same period were selected as the control group.The serum PD-1 levels before surgery were measured using an enzyme-linked immunosorbent assay(ELISA).The level of serum GINS4 mRNA before surgery was detected with real-time quantitative PCR.The relationship between serum GINS4 mRNA and PD-1 levels prior to surgery and the 5-year survival rate in the patients with gastric cancer were analyzed using the Kaplan-Meier method.Cox regression was used to analyze the factors affecting gastric cancer prognosis.Results The serum levels of GINS4 mRNA and PD-1 were significantly higher in the patients with gastric cancer than in the healty controls(P < 0.05).The serum levels of GINS4 mRNA and PD-1 in patients with poorly differentiated tissues in TNM stagesⅢand Ⅳ,and lymph node metastasis were significantly higher than those in patients with moderately/highly differentiated tissues,in TNM stageⅡ,and no lymph node metastasis(P < 0.05).The serum levels of GINS4 mRNA and PD-1,proportio of patients with TNM stagesⅢand Ⅳ,and the proportion of patients with lymph node metastasis in the death group were significantly higher than those in the survival group(P < 0.05).According to the results of Kaplan-Meier analysis,the 5-year survival rate of patients with gastric cancer with high serum expression levels of GINS4 mRNA and PD-1 was lower than that of patients with low expression levels of GINS4 m RNA and PD-1(P < 0.05).GINS4 m RNA and PD-1 levels were independent risk factors for death in patients with gastric cancer(P < 0.05).Conclusion The expression levels of serum GINS4 m RNA and PD-1 in patients with gastric cancer were higher than those of the controls,which were related to tissue differentiation,TNM staging,lymph node metastasis,and prognosis.
作者
唐尧宇
张丽柯
骆春艳
TANG Yaoyu;ZHANG Like;LUO Chunyan(Clinical Laboratory,The First People’s Hospital of Nanyang,Nanyang 473000,China;Cancer Laboratory,The First People’s Hospital of Nanyang,Nanyang 473000,China)
出处
《中国医科大学学报》
北大核心
2025年第2期156-160,166,共6页
Journal of China Medical University
基金
河南省医学科技攻关计划(LHGJ20200903)。
关键词
胃癌
GINS复合物4
PD-1
临床病理特征
预后
gastric cancer
GINS complex 4
PD-1
clinicopathological characteristics
prognosis
