摘要
目的了解郑州地区献血者戊型肝炎病毒(HEV)感染状况,为制定科学的血液筛查策略提供数据支撑。方法对2022年1—12月的献血者部分随机标本利用PCR技术检测HEV RNA,对阳性标本进行基因测序,并对献血者进行追踪随访。结果21311份标本中共检出HEV RNA阳性3(0.14‰)份,献血者均为男性。基因测序显示1份强阳性标本的基因型为4型,而其他2份因病毒载量低未能测序成功。对强阳性献血者进行的25次追踪显示,其转氨酶(ALT)在献血后d7显著升高,抗-HEV IgM和抗-HEV IgG转阳,d21 ALT恢复正常,d35 HEV RNA转阴,d482抗-HEV IgM和抗-HEV IgG仍持续存在。结论郑州地区献血者中存在HEV感染,应扩大筛查范围,全面了解HEV在献血者中的流行情况和基因型分布。
Objective To analyze the infection status of hepatitis E virus(HEV)among blood donors in Zhengzhou,so as to provide data support for formulating local blood screening strategies.Methods Random samples from blood donors from January to December 2022 were tested for HEV RNA using PCR technology.Reactive samples were sequenced for gene analysis,and the donors were followed up.Results Among 21311 samples,3(0.14‰)were reactive for HEV RNA,all of whom were male.Genetic sequencing results revealed that one strong positive sample was genotype 4,while sequencing failed for the other two due to low viral load.A follow-up of 25 strong positive donors showed that ALT significantly increased on day 7 after donation,anti-HEV IgM and anti-HEV IgG turned positive.On day 21,ALT returned to normal,and on day 35,HEV RNA turned negative.Notably,anti-HEV IgM and anti-HEV IgG persisted until day 482.Conclusion There is HEV infection among blood donors in Zhengzhou,and it is necessary to expand the screening scope to comprehensively explore the prevalence and genotype distribution of HEV among blood donors.
作者
赵红娜
魏跃光
闫路敏
涂甜甜
王淑敏
魏旖辉
王艺芳
赵磊
陈明军
ZHAO Hongna;WEI Yueguang;YAN Lumin;TU Tiantian;WANG Shumin;WEI Yihui;WANG Yifang;ZHAO Lei;CHENMingjun(Henan Red Cross Blood Center,Henan Blood Safety Research Institute,Zhengzhou 450000,China)
出处
《中国输血杂志》
2025年第1期13-18,共6页
Chinese Journal of Blood Transfusion
基金
河南省医学科技攻关计划联合共建项目(LHGJ20220262)。
