摘要
目的拟揭示少弱精子症验方石萆汤“特征峰成分药效”的谱效关系和药效物质基础。方法采用大鼠实验,其中石萆汤组以2倍人体等效剂量灌胃,空白对照组干预等体积生理盐水,持续给药7 d。使用UHPLC-QE-MS技术对石萆汤口服吸收后进入血液中的成分进行鉴定;通过网络药理学分析获得石萆汤的关键成分、关键靶点和富集通路;对关键入血成分与核心靶点分子予以对接,分析其“谱效”关系。结果在石萆汤中鉴定出异野樱素等41个化合物,并发现11个入血成分。石萆汤可能通过雌激素信号通路等通路来调节性激素水平,以及抗炎、抗氧化的作用,其中异野樱素、藏红花酸等6个成分可与核心靶点形成可视化分子对接。结论石萆汤可通过调节脂质代谢与内分泌失调治疗少弱精子症,其中异野樱素等黄酮类成分可能是石萆汤关键药效成分。
Objective To clarify the pharmacodynamic relationship and pharmacological substance basis of the characteristic peak component of Shibi decoction for oligoasthenozoospermia therapy.Methods The rat experiment was carried out in the study.The rats in the Shibi decoction group were orally administered with twice the equivalent human dose,while the rats in the control group was intervened with an equal volume of physiological saline.They were all continuously given medication for 7 days.The components of Shibi decoction that entered the bloodstream after oral absorption were identified by UHPLC-QE-MS technology,and the key components,key targets and enrichment pathways of Shibi decoction were obtained through network pharmacology analysis.Finally,a molecular docking analysis were performed between key blood components and core target molecules for determining spectrum-effect relationships.Results A total of 41 compounds in Shibi decoction were identified,including isosakuranetin,and 11 blood components were obtained.Shibi decoction might regulate sex hormones,anti-inflammatory and antioxidant through the estrogen signaling pathway.A visual molecular docking was formed between core targets and six components including isosakuranetin.Conclusion Shibi decoction may treat oligoasthenozoospermia by regulating lipid metabolism and endocrine disorders,and its key pharmacological ingredients are flavonoid components such as isosakuranetin.
作者
程宛钧
杨绘
冉添福
黄伟聪
张敏建
陈熵忠
潘日润
邓日森
江澍
Cheng Wanjun;Yang Hui;Ran Tianfu;Huang Weicong;Zhang Minjian;Chen Shangzhong;Pan Rirun;Deng Risen;Jiang Shu(People’s Hospital Affiliated to Fujian University of Traditional Chinese Medicine,Fuzhou 350004,Fujian,China;Fujian University of Traditional Chinese Medicine,Fuzhou 350122,Fujian,China)
出处
《中国男科学杂志》
CSCD
2024年第6期16-26,共11页
Chinese Journal of Andrology
基金
福建省自然科学基金(2020J011044)
国家自然科学基金面上项目(82074184)
2022年全国名老中医药专家传承工作室建设项目(国中医药人教函〔2022〕75号)
第四批福建省名老中医药专家学术经验继承工作项目(闽卫中医函〔2022〕554号)
福建中医药大学附属人民医院“肥胖与代谢疾病相关男性不育症的临床与基础研究”科研团队资助项目(闽人院(2023)25号)。
关键词
石萆汤
少精子症
弱精子症
代谢组学
网络药理学
分子对接模拟
大鼠
Shibi decoction
oligospermia
asthenozoospermia
metabolomics
network pharmacology
molecular docking simulation
rat
