摘要
菌种质量优劣直接影响产量甚至产业的发展前景,筛选出可用于大规模生产的优良菌株极其重要。通过筛选菌丝生长特性、酶活性、活性物质成分、营养物质,以及拮抗反应和ITS分子鉴定等几个方面,比较菌株间的差异。拮抗和分子鉴定结果表明,10个菌株间的相似性较高,筛选出H29菌株的菌丝生长速度、发酵生物量及胞内外多糖含量、胞内外三萜含量均较高,为优势菌株。相关性分析结果表明,菌丝生长速度、胞内多糖含量和漆酶酶活与发酵生物量呈显著正相关,并通过多元线性回归分析,建立了一套递进关键指标的快速评价菌株优劣的体系,其中菌丝生长速度对发酵生物量的影响最大。研究结果为优质黑木耳菌株的快速筛选及在生产中的推广应用奠定了基础。
The quality of the strains directly affects the yield and even the development prospects of their industry,and it is imperative to select good strains that can be used for large-scale production.Differences between strains were com-pared by screening several aspects such as mycelial growth characteristics,enzyme activity,active compound composi-tion,nutrients,antagonism and ITS molecular identification.The antagonistic and molecular identification results showed high similarity among the 10 strains,and strain H29 was screened as the dominant strain with higher mycelial growth rate,fermentation biomass,and intra-and extracellular polysaccharide and intra-and extracellular triterpene content.The results of correlation analysis showed that mycelial growth rate,intracellular polysaccharide content and laccase enzyme activity were significantly and positively correlated with fermentation biomass,and a set of recursive key indices for rapid evalua-tion of strain superiority and inferiority was established by multivariate linear regression analysis,in which mycelial growth rate had the greatest influence on fermentation biomass.The results of the study provided the basis for the rapid screening of high-quality Auricularia heimuer strains and the promotion of their use in production.
作者
卯明娟
洪鹏
刘迪
陆源添
侯立娟
MAO Mingjuan;HONG Peng;LIU Di;LU Yuantian;HOU Lijuan(Agricultural College of Yanbian University,Yanji 133002,Jilin,China;Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement/Institute of Vegetable,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,Jiangsu,China)
出处
《中国瓜菜》
北大核心
2025年第2期66-74,共9页
China Cucurbits And Vegetables
基金
延边州科技发展计划项目-基础研究(2024NS14)
中国东北地区菌物遗传调查及应用研究(延大横向﹝2019﹞第19号)。
关键词
黑木耳
活力检测
优良菌株
活性成分
Auricularia heimuer
Vitality testing
Dominant strains
Active ingredient
