摘要
目的探讨丹寿汤调控mTOR/S6K通路对人绒毛膜滋养层细胞HTR-8/Svneo自噬及增殖、凋亡的影响。方法将HTR-8/Svneo分为对照组、丹寿汤低(丹寿汤-L,100μmol/mL)、中(丹寿汤-M,150μmol/mL)、高剂量(丹寿汤-H,200μmol/mL)组、mTOR通路激活剂MHY1485(100 nmol/mL)组、丹寿汤-H+MHY1485组,处理24 h后,检测细胞增殖、凋亡、侵袭与迁移,观察自噬小体变化,检测LC3、p62、Beclin1 mRNA表达及mTOR/S6K通路相关蛋白的表达。结果与对照组相比,不同剂量的丹寿汤组增殖率、侵袭、迁移率、自噬小体/细胞质总面积、LC3、Beclin1 mRNA、p-mTOR/mTOR、S6K蛋白表达显著降低,凋亡率、p62 mRNA表达增加,组间差异有统计学意义,但MHY1485组增殖率、侵袭、迁移率、自噬小体/细胞质总面积、LC3、Beclin1 mRNA、p-mTOR/mTOR、S6K蛋白表达显著增加,凋亡率、p62 mRNA表达降低(均P<0.05);与丹寿汤-H组相比,丹寿汤-H+MHY1485组增殖率、侵袭、迁移率、自噬小体/细胞质总面积、LC3、Beclin1 mRNA、p-mTOR/mTOR、S6K蛋白表达显著增加,凋亡率、p62 mRNA表达降低(均P<0.05)。结论丹寿汤调控mTOR/S6K通路抑制人绒毛膜滋养层细胞HTR-8/Svneo自噬、增殖,诱导其凋亡。
Objective To investigate the effect of Danshou decoction on autophagy,proliferation and apoptosis of human villous trophoblast cell HTR-8/Svneo by regulating the mTOR/S6K pathway.Methods HTR-8/Svneo cells were divided into control,Danshou decoction low-dose(Danshou decoction-L,100μmol/mL),medium-dose(Danshou decoction-M,150μmol/mL),high-dose(Danshou decoction-H,200μmol/mL),the mTOR pathway activator MHY1485(100 nmol/mL),and Danshou decoction-H+MHY1485 groups.After 24 hours of treatment,cell proliferation,apoptosis,invasion,and migration were detected by using CCK-8,flow cytometry,Transwell,and wound healing assays.Autophagosome changes were observed,and the expression levels of LC3,p62,Beclin1 mRNA and mTOR/S6K pathway related proteins were detected.Results Compared with the control group,the proliferation rate,invasion rate,migration rate,the proportions of autophagosomes/total cytoplasmic area,LC3,Beclin1 mRNA,p-mTOR/mTOR,and S6K protein expression reduced,and the apoptosis rate and p62 mRNA expression increased in Danshou decoction group at different dosages.The proliferation rate,invasion rate,migration rate,the proportion of autophagosomes/total cytoplasmic area,LC3,Beclin1 mRNA,p-mTOR/mTOR,and S6K protein expression increased,and the apoptosis rate and p62 mRNA expression decreased in the MHY1485 group(all P<0.05).Compared with the Danshou decoction-H group,the proliferation rate,invasion rate,migration rate,the proportion of autophagosomes/total cytoplasmic area,LC3,Beclin1 mRNA,p-mTOR/mTOR,and S6K protein expression increased,and the apoptosis rate and p62 mRNA expression decreased in the Danshou decoction-H+MHY1485 group(all P<0.05).Conclusion Danshou decoction inhibits autophagy and proliferation,and induces apoptosis of human villous trophoblast cell HTR-8/Svneo by regulating the mTOR/S6K pathway.
作者
于喜乐
卫爱武
石少琦
崔天薇
宋红艳
Yu Xile;Wei Aiwu;Shi Shaoqi(Department of Reproductive Medicine the First Affiliated Hospital,Henan University of Traditional Chinese Medicine,Zhengzhou 450046,China;First School of Clinical Medicine,Henan University of Traditional Chinese Medicine,Zhengzhou 450046,China)
出处
《华中科技大学学报(医学版)》
北大核心
2025年第1期45-51,共7页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
基金
河南省自然科学基金资助项目(No.232300420058)。
