摘要
目的探讨宫颈癌组织中CCCH型锌指蛋白13(ZC3H13)、着丝粒蛋白H(CENPH)的表达及临床意义。方法收集2016年2月至2018年2月该院收治的302例宫颈癌患者作为研究对象。采用实时荧光定量PCR和免疫组织化学染色检测宫颈癌、癌旁组织中ZC3H13、CENPH mRNA和蛋白表达。分析ZC3H13 mRNA表达水平与CENPH mRNA表达水平的相关性。分析ZC3H13、CENPH表达与临床病理特征的关系。采用Kaplan-Meier生存曲线进行不同ZC3H13、CENPH表达情况患者生存情况的比较。采用多因素Cox回归分析宫颈癌患者预后的影响因素。结果与宫颈癌患者癌旁组织(1.18±0.25、1.03±0.20)相比,癌组织ZC3H13 mRNA(3.34±0.46)、CENPH mRNA(3.03±0.51)表达水平较高,差异均有统计学意义(t=71.697、63.445,P<0.05)。与宫颈癌患者癌旁组织[6.62%(20/302)、5.96%(18/302)]相比,癌组织ZC3H13[70.20%(212/302)]、CENPH[66.89%(202/302)]阳性率较高,差异均有统计学意义(χ^(2)=257.993、246.403,P<0.05)。宫颈癌中ZC3H13 mRNA表达水平与CENPH mRNA表达水平呈正相关(r=0.775,P<0.001)。FIGO分期ⅡA期、低分化、淋巴结转移的宫颈癌患者癌组织ZC3H13、CENPH阳性率明显高于FIGO分期ⅠA~ⅠB期、中高分化、无淋巴结转移的患者,差异均有统计学意义(P<0.05)。ZC3H13阳性患者与ZC3H13阴性患者的生存曲线比较,差异有统计学意义(Log-rankχ^(2)=9.785,P=0.002);CENPH阳性患者与CENPH阴性患者的生存曲线比较,差异有统计学意义(Log-rankχ^(2)=7.309,P=0.007)。FIGO分期、淋巴结转移、分化程度及宫颈癌组织中ZC3H13、CENPH是宫颈癌患者预后的影响因素(P<0.05)。结论宫颈癌ZC3H13、CENPH表达水平升高,且参与宫颈癌的发展,是影响宫颈癌预后的因素。
Objective To investigate the expression and clinical significance of CCCH-type zinc finger protein 13(ZC3H13)and centromere protein H(CENPH)in cervical cancer tissues.Methods A total of 302 cervical cancer patients admitted to the hospital from February 2016 to February 2018 were included as reasearch subjects.Real-time quantitative PCR and immunohistochemical staining were used to detect the mRNA and protein expression of ZC3H13 and CENPH in cervical cancer and adjacent tissues.The relationship between the expression levels of ZC3H13 mRNA and CENPH mRNA was analyzed.Additionally,the correlation between the expression of ZC3H13,CENPH and clinical pathological features was evaluated.Kaplan-Meier survival curves were used to compare the survival outcomes of patients with different expression levels of ZC3H13 and CENPH.Multivariate Cox regression analysis was performed to identify the prognostic factors affecting the survival of cervical cancer patients.Results Compared with adjacent tissues(1.18±0.25,1.03±0.20),the expression levels of ZC3H13 mRNA(3.34±0.46)and CENPH mRNA(3.03±0.51)in cancer tissues were significantly higher,with statistically significant differences(t=71.697,63.445,P<0.05).The positive rates of ZC3H13[70.20%(212/302)]and CENPH[66.87%(202/302)]were also higher in cancer tissues compared with adjacent tissues[6.62%(20/302),5.96%(18/302)],with statistically significant differences(χ^(2)=257.993,246.403,P<0.05).The expression levels of ZC3H13 mRNA and CENPH mRNA in cervical cancer were positively correlated(r=0.775,P<0.001).The positive rates of ZC3H13 and CENPH in cancer tissues were significantly higher in patients with FIGO stageⅡA,poorly differentiated tumors and lymph node metastasis compared with those with FIGO stageⅠA-ⅠB,moderately to well-differentiated tumors and no lymph node metastasis,with statistically significant differences(P<0.05).There was a statistically significant difference in the survival curves between ZC3H13-positive and ZC3H13-negative patients(Log-rankχ^(2)=9.785,P=0.002);similarly,there was a statistically significant difference in the survival curves between CENPH-positive and CENPH-negative patients(Log-rankχ^(2)=7.309,P=0.007).FIGO stage,lymph node metastasis,degree of differentiation and the expression of ZC3H13 and CENPH in cervical cance r tissues were identified as prognostic factors for cervical cancer patients(P<0.05).Conclusion The expression levels of ZC3H13 and CENPH elevate in cervical cancer,and both are involved in the disease progression,serving as factors that influence the prognosis of cervical cancer.
作者
程思
伍雯莹
胡静
CHENG Si;WU Wenying;HU Jing(Department of Women′s Health,Affiliated Maternity and Children′s Health Hospital of Hubei Polytechnic University,Huangshi,Hubei 435000,China)
出处
《检验医学与临床》
2025年第4期445-450,456,共7页
Laboratory Medicine and Clinic
基金
湖北省卫生健康委员会第三批联合基金项目(WJ2019H480)。
