摘要
目的探讨前蛋白转化酶枯草溶菌素9(proprotein convertase subtilisin/kexin type9,PCSK9)抑制剂对大鼠心肌缺血再灌注损伤的影响。方法选取Wistar雄性大鼠构建心肌缺血再灌注模型,分为假手术组(Sham组)和2 h、4 h、6 h、8 h、24 h、48 h组(固定缺血0.5 h,考察再灌注时间),每组6只,Western blot检测各组大鼠缺血心肌PCSK9蛋白水平,选取最佳再灌注时间;随后分为Sham组、心肌缺血再灌注组(I/R组)及心肌缺血再灌注+PCSK9抑制剂组(I/R+P组),每组18只;采用硫黄素S染色检测术后无复流(no reflow,NR)面积变化,伊文思蓝染色检测心肌缺血面积变化,TTC染色检测心肌梗死面积变化。结果8 h组PCSK9蛋白水平最高,选取再灌注时间为8 h进行后续实验;后续实验结果显示,与Sham组比较,I/R组和I/R+P组大鼠心脏的NR面积、心肌缺血面积、心肌梗死面积均升高(P<0.05);与I/R组比较,I/R+P组大鼠心脏的NR面积、心肌缺血面积、心肌梗死面积均降低(P<0.05)。结论PCSK9抑制剂可以减轻大鼠心肌缺血再灌注损伤。
Objective To investigate the effect of preprotein convertase subtilis 9(PCSK 9)inhibitor on myocardial ischemia-reperfusion injury in rats.Methods The male rats of Wistar were selected to construct myocardial ischemia-reperfusion model,and divided into sham group(Sham group)and 2 h,4 h,6 h,8 h,24 h,48 h groups(fixed ischemia of 0.5 h,to examine reperfusion time),with 6 rats in each group.The level of PCSK9 protein in ischemic myocardium of rats in each group were detected by Western blot and the optimal time for reperfusion was selected.Subsequently,the groups were divided into Sham group,myocardial ischemia reperfusion group(I/R group)and myocardial ischemia reperfusion+PCSK9 inhibitor group(I/R+P group),with 18 rats in each group.Sulfur S staining was used to detect the changes in the area of no recirculation(no reflow,NR)after surgery.The area of non-reflux(no reflow,NR)was detected by sulfur S staining after surgery.Evans blue staining was used to detect the changes in myocardial ischemic area,and TTC staining was used to detect the changes in myocardial infarction area.Results The level of PCSK9 protein was the highest in 8 h group,and the reperfusion time was selected as 8 h for subsequent experiments.Subsequent experimental results showed that the NR area,myocardial ischemic area and myocardial infarction area of rats in I/R group and I/R+P group were all increased,compared with Sham group(P<0.05).Compared with I/R group,NR area,myocardial ischemic area and myocardial infarction area of rats in I/R+P group were all reduced(P<0.05).Conclusion PCSK9 inhibitors can reduce myocardial ischemia-reperfusion injury in rats.
作者
李润红
舒敏
黄广伟
周海燕
李超
王冰
罗振华
李伟
LI Runhong;SHU Min;HUANG Guangwei;ZHOU Haiyan;LI Chao;WANG Bing;LUO Zhenhua;LI Wei(Department of Cardiovascular Medicine,the Affiliated Hospital of Guizhou Medical University,Guiyang 550004,Guizhou,China;Department of Emergency Medicine,Ziyang Central Hospital,Ziyang 641300,Sichuan,China;Nursing Teaching and Research Section,Sichuan Ziyang Stomatological Vocational College,Ziyang 641300,Sichuan,China;Central Laboratory,Guizhou Provincial People's Hospital,Guiyang 550002,Guizhou,China)
出处
《贵州医科大学学报》
2025年第2期205-211,共7页
Journal of Guizhou Medical University
基金
国家自然科学基金地区项目(81960047)。
