期刊文献+

基于指纹图谱和多成分含量测定结合化学模式识别的北沙参质量评价

Quality Evaluation of Glehniae Radix by HPLC Fingerprint and Multi-Component Quantitative Method Combined with Chemical Pattern Recognition
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摘要 目的建立北沙参高效液相色谱(HPLC)指纹图谱及多成分定量方法,结合化学模式识别,评价不同产地、不同带皮程度对北沙参质量的影响。方法采用HPLC法建立北沙参指纹图谱与7种指标成分含量的测定方法。采用指纹图谱相似度评价不同产地北沙参质量,应用聚类分析(HCA)、主成分分析(PCA)及正交偏最小二乘法(OPLS-DA)评价不同带皮程度北沙参质量,进行差异性成分分析。结果60批不同产地北沙参指纹图谱共有18个峰,指认了7个峰,各批次相似度在0.814~0.996。聚类分析将190批北沙参分为2类,显著区分残皮程度≤1/3及>1/3的样品,PCA与HCA结果一致,差异性质量标志物为人参炔醇、法卡林二醇。结论建立的HPLC指纹图谱和多成分含量测定方法操作简便,稳定可靠,可为北沙参的质量控制、整体评价及规范产地加工方式提供依据。 OBJECTIVE To establish the HPLC fingerprint and multi-component quantitative determination method of Glehniae Radix,and study the dispersion in quality of Glehniae Radix of different regions and residual root bark ratio by chemical pattern recognition.METHODS HPLC method was used to establish the fingerprint and determine the contents of seven active components.The chromatographic column was ShimNex CS C18(4.6 mm×250 mm,5μm),the mobile phase was methanol-0.4%phosphoric acid water,gradient elution was conducted at a volume flow rate of 1.0 mL·min-1,the column temperature was maintained at 30℃,and the detection wavelength was set at 204 and 248 nm.Fingerprint similarity was used to evaluate different regions of Glehniae Radix.Cluster analysis,principal component analysis and discriminant analysis were used to analyze the quality and the differential components of Glehniae Radix of different residual root bark ratio.RESULTS A total of 18 common peaks were found in the fingerprints of 60 batches of Glehniae Radix fingerprints from different regions,and seven peaks were identified,including facarindiol,panaxynol,xanthotoxin,bergapten,imperatorin,isocorallol and isoimperatorin.The similarity of 60 batches of samples was 0.814-0.996.The multi-component contents of the 190 batches of Glehniae Radix were divided into two groups by cluster analysis,and the samples with residual root bark ratio≤1/3 and>1/3 were significantly distinguished.The results of PCA and HCA were consistent.The different quality markers were panaxynol and facalindiol.CONCLUSION The established HPLC fingerprint and multi-component content determination method are simple,stable,and reliable,which can provide a basis for the quality control,overall evaluation and standardized processing methods of Glehniae Radix.
作者 耿莲 孔亚萍 王常顺 孙慧珠 苏建 王晓蕾 GENG lian;KONG Yaping;WANG Changshun;SUN Huizhu;SU Jian;WANG Xiaolei(NMPA Key Laboratory for Quality Monitoring and Evaluation of Traditional Chinese Medicine(Chinese Materia Medica),Hebei Institute for Drug and Medical Device Control,Shijiazhuang 050227,China)
出处 《中国药学杂志》 北大核心 2025年第1期77-85,共9页 Chinese Pharmaceutical Journal
基金 河北省药品监督管理局科技计划项目资助(2022ZC1023) 河北省科技厅科技研发平台专项资助(24462503D)。
关键词 北沙参 指纹图谱 多成分定量 法卡林二醇 人参炔醇 Glehniae Radix fingerprint multi-component content determination facalindiol panaxynol
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