摘要
为探究红豆越橘及金银花提取物对长波紫外线(UVA)辐射引起氧化应激的防护作用,制备了红豆越橘乙醇提取物和金银花多酚质量比为1∶1的复配物(简称复配物),建立了UVA诱导的肝癌细胞(HepG2细胞)氧化损伤模型,考察了复配物对UVA辐照后HepG2细胞的超氧化物歧化酶(SOD)活力、谷胱甘肽过氧化物酶(GSH-Px)活力、过氧化氢酶(CAT)活力、丙二醛(MDA)浓度和活性氧(ROS)含量的影响。结果表明,复配物通过激活抗氧化酶系和清除ROS来缓解由UVA辐照引发的HepG2细胞氧化应激损伤。HepG2细胞被半致死UVA辐照剂量(12.96 J/cm^(2))辐照后,与模型组相比,经低(50μg/mL)、中(100μg/mL)、高(200μg/mL)质量浓度复配物处理后的HepG2细胞存活率分别增加了15.26%、33.59%、66.15%,SOD活力分别增加了10.72%、32.43%、54.08%,CAT活力分别增加了16.48%、36.90%、116.20%,GSH-Px活力分别增加了52.97%、71.44%、117.14%,MDA浓度分别下降了17.12%、31.22%、59.41%,ROS表达水平显著下降。
In order to investigate the protective effects of extracts from Vaccinium vitis-idaea L.and Lonicera japonica on oxidative stress damage induced by long-wave ultraviolet(UVA)radiation,a UVA induced oxidative damage model of human hepatocellular carcinomas(HepG2 cells)was established to investigate the protective effect of a compound made from Lonicera japonica polyphenols and Vaccinium vitis-idaea L.ethanol extract(compound for short)with a mass ratio of 1∶1.The influence of the compound on superoxide dismutase(SOD)activity,glutathione peroxidase(GSH-Px)activity,catalase(CAT)activity,malondialdehyde(MDA)concentration and reactive oxygen species(ROS)content of HepG2 cells after UVA irradiation were analyzed.The results showed that the compound alleviated the oxidative stress damage of HepG2 cells induced by UVA irradiation through activating the antioxidant enzyme system and clearing ROS.Compared with those of model group,the survival rate of HepG2 cells after treatment with low(50μg/mL),medium(100μg/mL)and high(200μg/mL)mass concentration compounds was increased by 15.26%,33.59%and 66.15%,the SOD activity was increased by 10.72%,32.43%and 54.08%,the CAT activity was increased by 16.48%,36.90%and 116.20%,the GSH-Px activity was increased by 52.97%,71.44%and 117.14%,and the MDA concentration was decreased by 17.12%,31.22%and 59.41%,respectively,and ROS expression level decreased significantly.
作者
范伟佳
崔旭娜
刘嘉怡
王杰
王婷婷
樊梓鸾
FAN Weijia;CUI Xu'na;LIU Jiayi;WANG Jie;WANG Tingting;FAN Ziluan(College of Life Science,Northeast Forestry University,Harbin 150040,Heilongjiang,China;Key Laboratory of Forest Food Resources Utilization of Heilongjiang Province,Harbin 150040,Heilongjiang,China)
出处
《精细化工》
北大核心
2025年第3期570-576,共7页
Fine Chemicals
基金
国家自然科学基金项目(31170510)
中国博士后科学基金项目(2016M600239)
中央高校科学前沿与交叉学科创新基金项目(2572019BA09)
黑龙江省级大学生创新训练项目(S202310225282)
黑龙江省自然科学基金联合引导项目(LH2020C035)。
关键词
红豆越橘
金银花多酚
抗UVA辐射
活性氧
抗氧化酶
生物工程
Vaccinium vitis-idaea L.
Lonicera japonica polyphenols
anti-UVA radiation
reactive oxygen species
antioxidant enzyme
biological engineering
