摘要
目的:初步检测和分析长链非编码RNA酪氨酸激酶非催化区衔接蛋白1-反义RNA1(NCK1-AS1)在急性髓系白血病(AML)中的表达及临床意义。方法:选取江苏大学附属人民医院AML确诊患者89例和健康对照者23例,采用实时荧光定量聚合酶链式反应(RT-qPCR)方法检测两组骨髓标本中NCK1-AS1和NCK1的表达水平,分析NCK1-AS1的表达与患者的临床特征的关系,以及NCK1-AS1与NCK1之间的相关性。结果:NCK1-AS1表达水平在全部AML、非M3型AML和正常核型AML患者中均较对照组显著上调(P<0.01,P<0.05,P<0.01)。在非M3型AML中,NCK1-AS1高表达患者的血红蛋白含量低于低表达组(P=0.036),高表达组患者总体生存时间也较低表达组短(P=0.0378)。Cox多因素分析显示,NCK1-AS1表达是非M3型AML患者的一个独立预后因素(HR=2.392,95%CI:1.089-5.255,P=0.030)。与对照组相比,NCK1在全部AML、非M3型AML和正常核型AML中表达均显著上调(P<0.01,P<0.01,P<0.001)。NCK1-AS1与NCK1的表达存在一定的相关性(r=0.37,P=0.0058)。结论:NCK1-AS1在AML中高表达预示着AML患者预后不良。
Objective:To detect and analyze the expression and clinical significance of long non-coding RNA tyrosine kinase non-catalytic region adaptor protein 1-antisense RNA1(NCK1-AS1)in patients with acute myeloid leukemia(AML).Methods:89 AML patients and 23 healthy controls were included from the People’s Hospital Affiliated to Jiangsu University.Real-time quantitative polymerase chain reaction(RT-qPCR)was used to detect the expression levels of NCK1-AS1 and NCK1 in bone marrow samples.The relationship between the expression of NCK1-AS1 and the clinical characteristics of patients were analyzed,as well as the correlation between NCK1-AS1 and NCK1.Results:The expression level of NCK1-AS1 in all AML,non-M3 AML and cytogenetically normal AML(CN-AML)patients was significantly higher than that in the control group(P<0.01,P<0.05,P<0.01,respectively).In non-M3 AML,patients with high NCK1-AS1 expression had a significantly lower hemoglobin level than those with low NCK1-AS1 expression(P=0.036),furthermore,NCK1-AS1 high patients had shorter overall survival than NCK1-AS1 low patients(P=0.0378).Multivariate analysis showed that NCK1-AS1 expression was an independent adverse factor in patients with non-M3 AML(HR=2.392,95%CI:1.089-5.255,P=0.030).In addition,NCK1 expression was also significantly upregulated in all AML,non-M3 AML and CN-AML patients compared with controls(P<0.01,P<0.01,P<0.001,respectively).There was a certain correlation between NCK1-AS1 and NCK1 expression(r=0.37,P=0.0058).Conclusion:High expression of NCK1-AS1 in AML indicates poor prognosis of AML patients.
作者
程晨
徐子浚
夏培慧
闻向梅
马吉春
谷雨
于迪
钱军
林江
CHENG Chen;XU Zi-Jun;XIA Pei-Hui;WEN Xiang-Mei;MA Ji-Chun;GU Yu;YU Di;QIAN Jun;LIN Jiang(Central Laboratory,the Affiliated People’s Hospital of Jiangsu University;Zhenjiang Clinical Research Center of Hematology;Department of Hematology,the Affiliated People’s Hospital of Jiangsu University,Zhenjiang 212002,Jiangsu Province,China)
出处
《中国实验血液学杂志》
2025年第2期352-358,共7页
Journal of Experimental Hematology
基金
国家自然科学基金(82270179,81970118,81900163)
镇江血液学研究中心(SS2018009)
镇江社会发展基金会(SH2023022,SH2022026,SH2023022)
江苏大学医教协同创新基金项目(JDY2023008)。
