摘要
目的探讨紫外线辐照对人永生化表皮细胞(HaCaT)中酪氨酸激酶(JAK)-信号转导及转录激活因子(STAT)信号通路和炎症因子白细胞介素23(IL-23)、纤维连接蛋白1(FN1)和单核细胞趋化蛋白1(MCP-1)的影响。方法实验分为正常对照组(0 mj/cm 2)和3个不同剂量辐照组,辐照组分别采用紫外线(30、60、90 mj/cm 2)辐照HaCaT细胞,照射后24 h,通过光镜观察细胞形态的变化;采用酶联免疫吸附试验(Elisa)检测IL-23、FN1和MCP-1的含量;实时荧光定量PCR检测IL-23 mRNA、FN1 mRNA和MCP-1 mRNA的表达水平;蛋白免疫印迹实验(Western-blot)检测TYK2、p-STAT4和p-STAT6的表达情况。结果与正常对照组相比,HaCaT细胞经不同剂量紫外线照射24 h后,IL-23、FN1和MCP-1的含量均增加(P<0.05),且与辐照剂量呈剂量依赖性;IL-23 mRNA、FN1 mRNA和MCP-1 mRNA表达水平均升高(P<0.05),且与辐照剂量呈剂量依赖性;TYK2、p-STAT4和p-STAT6蛋白表达均升高(P<0.05),且上调程度与照射剂量呈剂量依赖性。结论JAK-STAT信号通路及炎症因子IL-23、FN1、MCP-1参与紫外线照射HaCaT细胞损伤的调控。
Objective To investigate the role of JAK-STAT pathway and IL-23、MCP-1 and FN1 in HaCaT cells with UV irradiation.Methods HaCaT cells were irradiated with different dose of UV(30、60、90 mj/cm 2),after 24 h,microscope observed cell morphology.The enzyme linked immunosorbent assay(Elisa)to measure the levels of IL-23,FN1 and MCP-1 in the culture lysate of HaCaT cells;the levels of IL-23 mRNA,FN1 mRNA and MCP-1 mRNA was detected by qRT-PCR,the levels of TYK2,p-STAT4 and p-STAT6 were measured by Western-blot.Results Compared with the control group,the levels of IL-23,FN1 and MCP-1 increased,the levels of IL-23 mRNA,FN1 mRNA and MCP-1 mRNA were increased,the levels of TYK2,p-STAT4 and p-STAT6 were increased by the increase doses of UV.Conclusion JAK-STAT pathway and IL-23,FN1 and MCP-1 play a role in HaCaT with UV irradiation.
作者
喻晶
张宜
朱以良
陈芳
刘琴
YU Jing;ZHANG Yi;ZHU Yiliang;CHEN Fang;LIU Qin(Laboratory Animal Room,General Hospital of Central Theater Command,Wuhan 430070,China)
出处
《西部医学》
2025年第3期338-342,共5页
Medical Journal of West China
基金
中部战区总医院育英计划项目(ZZYFH202117)。
