摘要
目的:分析新疆维吾尔自治区高危型人乳头瘤病毒16型(HPV16)E5基因突变情况,探索主要突变位点与不同HPV感染类型、不同遗传背景、不同宫颈病变的相关性。方法:收集111例HPV分型检测结果为HPV16单一阳性,经9个月随访再次检测,分型仍为HPV16单一阳性患者的宫颈脱落细胞,提取细胞DNA,扩增纯化E5基因片段进行测序,与标准基因序列进行比对,寻找E5基因突变位点。结果:HPV16 E5基因高频突变位点为A193G、A130C、C142A,突变率在持续性感染与一过性感染总体分布差异无统计学意义(p>0.05)。一过性感染、持续性感染中A130C、A193G突变率在不同民族、不同宫颈病变的分布差异无统计学意义(p>0.05),C142A突变率在汉族女性与维吾尔族女性中的分布差异有统计学意义(p=0.012)。结论:HPV16 E5基因A193G、A130C、C142A突变与持续性感染无关;在不同感染类型下,A193G、A130C、C142A突变与宫颈病变发展无关;A130C、A193G突变无民族差异性,维吾尔族女性更易发生C142A突变。
Objective:To analyze the mutation of high-risk human papillomavirus type 16(HPV16)E5 gene in Xinjiang Uygur Autonomous Region,and explore the correlation between major mutation sites and different HPV infection types,different genetic backgrounds,and different cervical lesions.Methods:A total of 111 HPV typing patients are collected with single positive HPV16 positive results,and after 9 months of follow-up,the cervical exfoliated cells of the single positive HPV16 patients are still classified,the cell DNA is extracted,the E5 gene fragment is amplified and purified for sequencing,and the E5 gene sequence is compared with the standard gene sequence to find the E5 gene mutation site.Results:The high-frequency mutation sites of HPV16 E5 gene are A193G,A130C and C142A,and there is no significant difference in the mutation rate between persistent infection and transient infection(p>0.05).There is no significant difference in the distribution of A130C and A193G mutation rates in different ethnic groups and cervical lesions in transient infection and persistent infection(p>0.05),but there is a significant difference in the distribution of C142A mutation rate among Han women and Uygur women(p=0.012).Conclusion:Mutations in HPV16 E5 gene A193G,A130C,and C142A are not associated with persistent infection;under different types of infection,A193G,A130C,and C142A mutations are not associated with the development of cervical lesions;there is no ethnic difference between A130C and A193G mutations,and Uygur women are more likely to develop C142A mutations.
作者
胡金桂
王琳
朱开春
HU Jingui;WANG Lin;ZHU Kaichun(Medicine School,Shihezi University,Shihezi 832000,China;Department of Gynecology,People’s Hospital of Xinjiang Uygur Autonomous Region,Urumqi 830000,China;Laboratory of Obstetrics and Gynecology,People’s Hospital of Xinjiang Uygur Autonomous Region,Urumqi 830000,China)
出处
《生物化工》
2025年第1期128-131,145,共5页
Biological Chemical Engineering
基金
新疆维吾尔自治区自然科学基金项目(2022D01C103)。
关键词
人乳头瘤病毒
基因突变
持续性感染
一过性感染
human papillomavirus
genetic mutations
persistent infection
transient infection
