摘要
利用套叠PCR技术将α factor信号肽基因插入pAO815之EcoRⅠ位点,构建成分泌型表达载体pAO815α A.在不改变原克隆位点EcoRⅠ的条件下,借助pAO815之第873位的HindⅢ位点,将pAO815AOX1启动子873 940片段连同α factor信号肽基因序列(246bp)插入pAO815之HindⅢ EcoRⅠ之间,构建成含α factor信号肽基因的分泌型表达载体pAO815α A.
The pAO815αA of secrete expression vector was constructed with αfactor signal peptide gene inserted into EcoR Ⅰ site of pAO815 by overlap extension of PCR technique,which 873th940th bps fragment of pAO815 AOX1 promoter with αfactor signal peptide gene was inserted into Hind Ⅲ/EcoR Ⅰ site and a kind of pAO815 containing αfactor signal peptide gene was constructed under nonchanging previous EcoR Ⅰ cloning site.
出处
《生命科学研究》
CAS
CSCD
2002年第4期310-313,共4页
Life Science Research
基金
国家自然科学基金资助项目(30060033)
