摘要
目的 研究超临界流体CO2 萃取白芷中欧前胡素的提取工艺 ,并用高效液相色谱法测定欧前胡素的含量。方法 在InertsilODS 3柱上 ,以甲醇 水 (65∶35)为流动相 ,流速 :1 .5mL·min- 1 ,检测波长 :2 4 8nm ,柱温 :40℃。结果 欧前胡素浓度在 0 .0 4 1 5～ 0 .1 66mg·mL- 1 范围内呈线性关系 (r=0 .9998) ;加样平均回收率 :99.1 2 % ,RSD为 2 .73 %。结论 采用SFE萃取白芷中欧前胡素的提取工艺速度快 ,HPLC测定欧前胡素的含量精确。
OBJECTIVE: To investigate the extraction technology of imperatorin in Angelica dahurica by supercritical fluid, and to determine the content of imperatorin by HPLC. METHODS: The inertsil ODS-3 column was used with a mobile phase of methyl alcohol-water(65: 35). The flow rate was 1. 5 mL · min-1. The detection wavelength was at 248 nm. The column temperature was 40°C. RESULTS: The calibration curve of imperatorin was linear over the range of 0. 041 5 ∼ 0. 166 mg·mL-1 r = 0. 999 8). The average recoveries were 99. 12 % with RSD 2. 73 %. CONCLUSION: The extraction technology was rapid, and the HPLC assay was accurate and reproducible.
出处
《中国药学杂志》
EI
CAS
CSCD
北大核心
2003年第2期99-100,共2页
Chinese Pharmaceutical Journal
基金
贵州省卫生厅 2 0 0 0年优秀青年科技人才基金课题
