摘要
目的 研究葛根素 (puerarin)对缺氧性血管内皮细胞凋亡的影响。方法 用NaCN合并无糖培养基造成培养的牛主动脉内皮细胞 (BAECs)缺氧 ;用台盼蓝染色、末端脱氧核苷酸转移酶介导的dUTP缺口末端标记 (TUNEL)、流式细胞仪计数和Hoechst3 3 3 42荧光染色法观察细胞受损和凋亡情况 ;用免疫细胞化学染色法观察细胞内Caspase 3的表达情况。结果 NaCN合并无糖培养基可引起血管内皮细胞凋亡。葛根素可显著减少缺氧性内皮细胞凋亡 ,并对Caspase 3的表达有明显抑制作用。结论 葛根素对缺氧条件下的血管内皮细胞有保护作用 ,此作用至少部分通过抑制Caspase
Aim To study the effect of puerarin on vascular endothelial cells apoptosis induced by chemical hypoxia ischemia in vitro . Methods The chemical hypoxia ischemia model was performed by treating cultured bovine aortic endothelial cells (BAECs) with NaCN in glucose free medium. Cell viability was determined by trypan blue staining. Cell apoptosis was defined by terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL), flow cytometry and Hoechst 33342 staining. The expression of Caspase 3 in endothelial cells was detected by immunocytochemical method. Results Chemical hypoxia ischemia was shown to initiate bovine aortic endothelial cell apoptosis. Puerarin (0 5-3 mmol·L -1 ) was found to inhibit endothelial cell apoptosis effectively, and reduce the expression of Caspase 3 significantly. Conclusion Puerarin can protect apoptotic endothelial cells induced by chemical hypoxia ischemia markedly and the effect was performed partly by decreasing Caspase 3 expression.
出处
《药学学报》
CAS
CSCD
北大核心
2003年第2期103-107,共5页
Acta Pharmaceutica Sinica
