摘要
对不同类型的黄瓜品种的气孔形态进行了扫描电镜观察 ,发现需水量不同的黄瓜品种气孔形态特征有很大的差异。保卫细胞表观差异比较明显 ,气孔的密度、大小及开张度均有显著的差异 :亚洲类型的黄瓜品种气孔密度较欧洲类型与欧亚杂交品种大 ,气孔的保卫细胞的大小为欧洲品种 >欧亚杂交品种 >亚洲品种。气孔周围的形态特征表现为 :亚洲品种保卫细胞角质褶皱较多 ,起伏较大 ,气孔深深地陷于褶皱中 ;欧洲品种黄瓜气孔保卫细胞周围角质则较平坦 ,保卫细胞突出于表皮细胞之上 ;欧亚杂交品种的气孔大小与形态介于上述二者之间。土壤含水量的不同也影响黄瓜叶片气孔的形态特性 ,随着土壤含水量的减少 ,黄瓜叶片单位面积的气孔密度增加 ,保卫细胞的大小变小 ,气孔开展度增加。
The characteristics of leaf stoma of different cucumber ecotypes were studied by scan electron microscope. The leaf selected for scanning electron microscopy were 1mm2, were fixed in 3mml, 1mol/L gluteraldehyde for 24 h, rinsed three times with cacodylate buffer (pH 7.2), then postfixed in osmium tetroxide for 2 h. Samples were removed from osmium tetroxide, washed there times in the cacodylate buffer, and dehydrared in a graded series of ethanol, later embedded in Spur's epoxy resin, attached to uncoated slide and stained with epoxy tissue stain.Then Sputter-coated with gold in a sputter coater. Sample were cut into halves, one was used for the observation of face leaf, the other was used for reversed leaf.Leaf surface morphology was examined with scanning electron microscope S-530. The stoma density was observed in low multiple, others were with high multiple.10 view fields were observed.The results were as followed: Difference of leaf stoma structure in different cucumber ecotypes was significant.The stomata density of Asia type(Xintaimici and Tiancuilv) is higher than that of European ecotypes(Marianna and Deltstar) and European-Asia hybrid ecotypes(Yushi 3 and 97W-19).The guard cell length of European ecotypes is longer than that of others ;there are many obvious deep concave-convex cubicula drapes around the guard cell and depressed center of the drapes in Asia ecotypes stoma,which will store CO 2 and H 2O for photosynthesis and reduce the transpiration;For European ecotypes (Marianna),the stoma stands out the flat cuticular ,which allow CO 2 and H 2O in and out freely , as for Deltstar, there is obvious protuberance around the stomata,the cuticular around the stomata is less flat than that of Marianna.As for European-Asia hybrid ecotypes Yushi 3 and 97W-19, the leaf characteristics are between the two ecotypes above.With the soil moisture decreased, the stomata density increased, but the guard cell size is smaller, and the stomata aperture is larger.
出处
《生态学报》
CAS
CSCD
北大核心
2003年第1期199-204,共6页
Acta Ecologica Sinica
基金
国家自然科学基金项目 ( 39830 2 30 )~~
